Research group: Head of the ass. PhD professor Matyushko D.N.

Responsible performer: Associate Professor, PhD Amanova D.E.

Performers:

1. Master of IT medicine Bakytzhan A.
2. PhD Bolatbekova A.A.
3. Master's degree Silishchev D.A.

A survey was conducted among 154 students (6th- and 7th-year students and 1- to 3-year residents) and 64 teachers in surgical disciplines at three medical universities in the Republic of Kazakhstan. Traditional forms of assessment (testing and oral examination) are perceived as partially objective (Me=2-2.1), whereas Mini-CEX and AXIS are perceived as highly objective (Me=1.35 and Me=1.51, respectively). The concept of the KazMEE digital platform integrates four assessment methods (testing, oral exam, Mini-CEX, AXIS), an automated calculation formula, and a modular structure (authorization, content, testing, evaluation, analytics). A prototype of the educational interface and a technical specification for software implementation have been created. A pilot test of the platform was conducted among 125 students and 40 teachers, with an average satisfaction score of 4.38 on the Likert scale.

A convenient digital tool has been developed for integrated training and objective assessment of surgical competencies, combining testing, oral examination, Mini-CEX, and AXIS in a single system with automatic calculation of results and analytics. The platform increases transparency of control, reduces assessment subjectivity, and simplifies the organization of current and final certification, ensuring high user satisfaction.

1 article was published and indexed in the Scopus and Web of Science databases, Amanova, D., Matyushko, D., Bakytzhan, A., Bolatbekova, A., Sharapatov, Y., & Silishev, D. (2026). Assessment of perceived objectivity of clinical evaluation methods in surgical education: an approach using a digital platform.// Future Science OA, 12(1). doi.org (Scopus 82%, Q1)

1 article in the journal KKSON Amanova D.E., A.D. Bakytzhan, E.S. Zhunusov, D.N. Matyushko The use of electronic educational platforms for monitoring surgical competencies in medical universities - Medicine and Ecology. - 2024. - No. 2 (111). - pp.85-93. (reviewed by COXON list 1);

The monograph Matyushko D.N., Amanova D.E. Electronic learning and assessment platforms in the practice of medical universities in the light of modern educational concepts was published. Approved by the Protocol of the Senate of the National Academy of Sciences of the Russian Academy of Sciences No. 2 dated 09/25/2025 ISBN 978-601-040585-7.- Printing house "Arko"-2025. 66 p.

Received 1 copyright certificate, 2 patents, three implementation certificates, 5 abstracts in the materials of international scientific conferences.

Research group:

1. Riklefs Viktor - PhD, Vice-Rector for Academic Work, Karaganda Medical University, The Principal Investigator. https://orcid.org/0000-0002-5127-1236.
2. Bukeyeva A.S. - PhD, Head of the Department of Strategic Development and Quality Management, Karaganda Medical University, https://orcid.org/0000-0001-8842-9175.
3. Riklefs V.V. - specialist in the Department of Academic Work, Karaganda Medical University, https://orcid.org/0000-0002-2401-8868.
4. Barikova A.R. – assistant professor, E.A. Buketov University of Karaganda.https://orcid.org/0000-0002-5362-5560.
5. Umurkulova M.M. - PhD-student, E.A. Buketov University of Karaganda, specialty "Psychology of education"https://orcid.org/0000-0001-8469-6989.
6. Kassatova A.M. - PhD-student, L.N. Gumilyov Eurasian National University.https://orcid.org/0000-0002-4603-819X.
7. Omarbekova N.K. – Master of Pedagogical Sciences, Assistant Professor of the Department of Informatics and Biostatistics.https://orcid.org/0000-0002-0764-7312.
8. Ospanova M.B. – Master of Pedagogical Sciences, psychologist, assistant professor of the Department of Kazakhstan’s History and socio-political disciplines. https://orcid.org/0000-0003-2926-4627.
9. Drobchenko Е.А. – master student of 7M10123 Biomedicine. https://orcid.org/0000-0001-9291-7585.

Psychophysiological parameters of medical students with the use of different educational technologies were monitored and a comprehensive assessment of the effectiveness of different educational technologies that develop flexible skills in terms of psycho-emotional state andlearning outcomes was given. T

The educational technologies closest to the real situation cause higher psychophysiological tension. The combination of soft physiological mode of learning and detailed feedback leads to learning efficiency and mastery of all required flexible skills by the learners. The effectiveness of learning depends not only on the educational technology used, but also on the type of task at hand. Different learning tasks require different flexible skills that can be mastered more or less effectively when used. Individual responses are most pronounced in the types of learning that are closest to practice. External academic stress, perceived psychologically, leads to an overreaction to peak loads during learning, reducing its effectiveness. The author's certificate for the developed method of adaptive learning was obtained

The economic and educational effectiveness of different learning trajectories stimulating the development of flexible skills in medical education was evaluated. Approaches to optimize costs while maintaining high educational efficiency are proposed.

The educational effectiveness of the implementation of adaptive learning methods has been analyzed. Based on the results of two-stage expert evaluation of Delphi methods, the draft methodological recommendations for creating an adaptive environment of higher education of different teaching methods are compiled.

The primary project application area is the secondary and higher education. The projects results will benefit the school and university teachers, administration of schools and universities, as well as regional Departments of Education, Ministry of Education and Science, Ministry of Healthcare.

Main publications on the project topic

1. Психометрический тест как индикатор профессиональной пригодности абитуриентов / G. Kemelova, V. Riklefs, A. Kassatova, D. Aimbetova // «Росмедобр-2022. Инновационные обучающие технологии в медицине». –Moscow, 2022. – https://www.rosmedobr.ru/rosmedobr2022/thesis/64325/
2. V. Riklefs, M. Umurkulova, A. Bukeyeva, V. Riklefs. Психолого-педагогическоеобоснование необходимости развития навыков конвергентного обучения у студентов напримере медицинского и многопрофильного университетов Казахстана // KhabarshyBulletin of the National Pedagogical University named after Abay, series “Psychology”. –2021.– №3.– P. 60-65. https://amee.assetbank-server.com/assetbankamee/action/viewAsset?id=3113&index=12&total=29&view=viewSearchItem.
3. Psychometric testing and adaptive learning as prerequisite to successful adaptation to higher medical educatio / V. Riklefs, A. Bukeyeva, V. Riklefs // Abstract Book, Annual Conference of Association for Medical Education in Europe (AMEE). – Lyon, 2022. – P. 179. – https://www.rosmedobr.ru/rosmedobr2022/thesis/64325/.
4. F. Mindubayeva, V. Riklefs, Y. Drobchenko, A. Bukeyeva. Опыт использования новыхобразовательных технологий при модульном обучении студентов в Карагандинскоммедицинском университете / Abstract Book, VIII International educational and methodological conference “Medical education: the choice of the 21st century generation”, 2022. - Kazan: Kazan State Medical University. P. 50-55
5. Stress response in medical students to different fidelity of simulation: impact for curriculum designers / V. Riklefs, Y. Drobchenko, A. Bukeyeva, G. Kemelova, D. Aimbetova // Abstract Book, Annual Conference of Association for Medical Education in Europe (AMEE). –Glasgow, 2023. – P. 940-941
6. The master's thesis “Analysis of the effectiveness of educational technologies from the perspective of the development of soft skills and the level of psychophysiological stress in students” was defended by E.A. Drobchenko, part of the research group.

Research group:

1. Laryushina E.M. - scientific supervisor of the project, candidate of Medical Sciences, Professor, Head of the Department of Internal Diseases of NJSC "Medical University of Karaganda" Hirsch index 4, author's identifier in Scopus: 57196123971, ORCID 0000-0002-9590-690X.
2. Turgunov E.M. - senior Research Fellow, doctor of Medical Sciences, Professor, Vice-Rector for Scientific and Clinical Work of NJSC "Medical University of Karaganda". H-index 2, Scopus author ID: 57193721109, ResearcherID: R-2915-2016, ORCID: 0000-0002-6486-3847
3. Marchenko A.B. - senior Researcher, PhD, Leading Researcher, SL NJSC "Medical University of Karaganda" Hirsch index 2, Scopus author ID: 57193758669, ORCID 0000-0002-0325-7659.
4. Samoilova N.M. - junior researcher, PhD doctoral student of the 2nd year of study in the specialty 8D10100 "Medicine", gastroenterologist, assistant of the Department of Internal Diseases of NJSC "Medical University of Karaganda". ORCID: 0000-0003-0461-7078
5. Shkreba A.P. - junior researcher, resident of the 2nd year of study in the specialty "General Surgery", endoscopist.
6. Zhumadilova Zh,A. - junior Research Fellow:, Master of Science, Research Fellow, SL NJSC "Medical University of Karaganda". ORCID: 0000-0003-3227-1692.
7. Seisenbekova A.K. - junior researcher, gastroenterologist, assistant of the Department of Internal Diseases of NJSC "Medical University of Karaganda".

The degree of achievement of set goals and objectives is high. The tasks are carried out strictly according to plan and comply with the technical specifications of the contract.

The results of the examination (clinical, laboratory studies, data from endoscopic, morphological, radiological examination methods) of 50 study participants (40 patients with IBD, 10 respondents in the control group) included in the study for 2022 were entered into the database.

84 (69 with IBD, 15 control group respondents) study participants, after a preliminary telephone conversation, were invited to the medical university clinic, a clinical examination of 84 patients with IBD was carried out, including: collection of complaints, data on the medical history and life, physical examination of the patient, analysis of the results of previous laboratory, instrumental examinations, hospitalizations, treatment plans, examination results (clinical, laboratory studies, data from endoscopic, morphological, radiation examination methods),entered into the database, biological materials were collected, the level of TMAO in the blood serum and LPS protein was determined, the results of a general blood test, the level of total protein, albumin, CRP in the blood serum, fecal calprotectin were obtained at the National Laboratory Astana Private Institution. stool samples were delivered

Statistical processing of the research results using the IBM SPSS Statistics 22 program has begun.

3 employees of VNK received membership in the international organization ECCO in 2023 (European Society for the Study of Crohn's Disease and Ulcerative Colitis): Laryushina E.M., Samoilova-Bedych N.M., Seisenbekova A.K

The results of the research were presented in the form of reports at conferences.

Being fundamental in nature, the study will answer the questions posed and decide whether there is a need for further work in this direction, whether it is promising, and whether alternative methods of correcting the condition are possible for patients with IBD.This will make it possible not only to theoretically approach the choice of diagnostic and treatment strategies, but also to expand the capabilities of the clinician, which will subsequently lead to earlier diagnosis, and therefore more productive and effective treatment of patients

Information for potential users

researchers, GPs, gastroenterologists, resuscitators, general practitioners, patients with IBD..

Dissemination of work results: publications in foreign and domestic publications, implementation in the educational process when teaching theoretical and clinical disciplines.

Main publications on the project topic

1. Prevalence and risk factors of IBD (according to the register of the Karaganda region), Zhirenbaeva A.M., Igenbekova A.T., VIth Central Asian International Scientific and Practical Conference on Medical Education “Education of the Future: Wind of Change”, Karaganda, NAO MUK , Karaganda, 20-21.04.23 (poster report);
2. Epidemiological assessment and factors of inflammatory bowel diseases in patients of Central Kazakhstan, Samoilova-Bedych N.M., 25th International Medical Slavic-Baltic International Forum “St. Petersburg-Gastro-2023-ON-Line”, May 18, 2023 ( oral report);
3. New facets of studying intestinal metabolites: TMAO and IBD. Laryushina E.M., SamoilovaBedych N.M., Republican conference with international participation dedicated to “World Digestive Health Day”, Astana, June 2, 2023 (oral report)
4. TMAO (trimethylamine N-oxide): a metabolite as a biomarker for inflammatory bowel disease? Samoilova-Bedych N.M., Laryushina E.M., Seisenbekova A.K., Marchenko A.B., Igenbekova A.T., Zhirenbaeva A.M. TWENTY-NINTH UNITED RUSSIAN GASTROENTEROLOGY WEEK, Moscow, RF, September 26-28, 2023 (oral report, publication of thesis);
5. TRIMETHYLAMINE-N-OXIDE AND ACTIVITY OF INFLAMMATORY BOWEL DISEASES, N.M. Samoilova-Bedych, E.M. Laryushina, N.V. Kabdulina, A.M. Zhirenbaeva, VIII Central Asian Gastroenterological Week, Almaty, October 5-7, 2.23 (poster presentation)
6. Accepted for publication and approved oral presentation: Trimethylamine N-oxide: a metabolite associated with Crohn's disease? Samoilova-Bedych N.M., Laryushina Y.M., Marchenko A.B., Seisenbekova A.K., Bangkok, Thailand, Asian Pacific Digestive Week, December 6-9, 2023.

1. Ibraeva L.К. - Doctor of Medical Sciences, Professor of the Department of Internal Diseases NC JSC" ҚМУ " – project manager, https://orcid.org/0000-0002-9917-0258
2. Omarkulov B. K. -: Сandidate of Medical Sciences, Associate Professor, Director of theInstitute of Public Health an d Professional Health NAO "ҚМУ". https://orcid.org/0000-0002-3955-4452
3. Zharylkasyn Zh.Zh. - Candidateof Medical Sciences, Associate Professor, Head of theresearch Sanitary and hygienic Laboratory NC JSC "ҚМУ".https://orcid.org/0000-0001-5432-4842
4. Grebeneva O.V. - Doctor of Medical Sciences, Associate Professor, Chief Researcherof the Sanitary and Hygienic Research Laboratory NC JSC "ҚМУ" https://orcid.org/0000-0002-1758-3009
5. Rybalkina D.H. - Doctor of Medicine, Associate Professor of the Department of InternalDiseases NC JSC "ҚМУ", https://orcid.org/0000-0002-2041-1259
6. Bacheva I.V. - PhD, Associate Professor of the Department of Internal Diseases NC JSC"ҚМУ"https://orcid.org/0000-0002-5576-8637
7. Shadetova A.Zh. – Candidate of Biological Sciences, leading researcher of the researchsanitary and hygienic laboratory NC JSC "ҚМУ" https://orcid.org/0000-0003-3029-855X
8. Rusyaev M.V.-Master's degree, research associate of the Research Sanitaryand hygienicLaboratory NC JSC "ҚМУ". https://orcid.org/0000-0003-3212-4211
9. Alekseyev A.V.- PhD, Research Associate of the Sanitary and Hygienic Research Laboratory NC JSC "ҚМУ", https://orcid.org/0000-0002-8988-3609
10. Sabirov Zh.B. - PhD, researcher at the Sanitary and Hygienic Research Laboratory NC JSC "ҚМУ".

1. The main adverse occupational factors are as follows:

- For MRI medical staff - electromagnetic fields (EMF) of radio frequency 30-300 MHz by the magnetic component (at 41.2% of workplaces, 4.6-16.2 A/m) and industrial frequency EMF by the electric component (up to 1.3 kV/m at 47.1% of workplaces), in combination with elevated noise levels (up to 64.3 dBA at 50% of workplaces) and unfavorable microclimate conditions (at 58.9% of workplaces, air temperature exceeds the maximum permissible level by 4.6°C; at 47.1% of workplaces, relative air humidity drops to 33.1%; low air movement speed -0.02-0.08 m/s).

- For ultrasound (US) medical staff - industrial frequency EMF by the electric component (at 50% of workplaces, 0.55-1.1 V/m) in combination with elevated noise levels (at 61.1% of workplaces, ranging from 51.6 to 63.1 dBA), inadequate lighting (at 83.3% of workplaces, less than 153 lux), and unfavorable microclimate conditions (at 77.8% of workplaces, air temperature exceeds the maximum permissible level by 6.5°C; at 66.7% of workplaces, relative air humidity drops to 30.1%; low air movement speed - 0.03-0.08 m/s)

- For ophthalmology medical staff - increased noise levels (at 33.3% of workplaces, ranging from 50.4 to 57.9 dBA), insufficient lighting (at 74.1% of workplaces, less than 252 lux), and unfavorable microclimate conditions (at 92.6% of workplaces, air temperature exceeds the maximum permissible level by 6.5°C, and relative air humidity is below the maximum permissible level by 17.2%; low air movement speed - 0.015-0.08 m/s).

2. The dose loads of chemical substances on the bodies of medical staff working in MRI, ultrasound, and ophthalmology clinics due to inhalation at workplaces of low hazard for the development of specific diseases.

Based on the results of the study of the quality of life of medical staff using the WHOQOL-BREF questionnaire, it was found that the overall quality of life of the medical staff included in the study is rated as "high." Medical personnel, regardless of their field of activity, demonstrate high subjective well-being, with respondents reporting that their basic needs and quality of life are satisfactory.

Medical personnel in all three study groups exhibit sufficient physical and psychological well-being and a subjective sense of success in social interactions. Although the indicators of the "Physical and Psychological Well-being" domain of quality of life for MRI medical staff were 82.0±2.0%, for ophthalmological staff - 79.2±3.1%, which are higher than the corresponding domain levels for ultrasound staff, which were 68.8±2.8%, with statistical significance levels of p=0.0004 and 0.008, respectively.

However, 7.3% of respondents reported a slightly decreased quality of life and possible negative emotional states (anxiety, tension, emptiness, and boredom) with a sense of monotony in their daily lives and high workloads.

Medical staff working with MRI and ultrasound equipment subjectively note the presence of factors significantly affecting their work performance, such as various frequency EMF, noise, personal protective equipment, inadequate lighting, and suboptimal air temperature.

The results of psychophysiological research indicate that mild depression may develop due to the work-related stress, such as night shifts and the responsibility for accurate diagnostics among MRI specialists. On the other hand, a high level of depression may be associated with professional burnout among ultrasound specialists and ophthalmologists.

Medical staff working in MRI clinics demonstrate a slower aging process, a younger actual age, and lower biological age compared to staff in ultrasound and ophthalmology clinics. This may be due to intermittent exposure to low EMF values and can be considered a stimulating and favorable effect on the human body, provided that safety requirements for work operations are strictly observed.

Lower average values of attention, verbal short-term memory for words, and visual shortterm memory for numbers were found among MRI specialists compared to the control group. This indicates a high intensity of risk factors affecting their psychofunctional activity, leading to fatigue. The mental work productivity and accuracy among MRI specialists were also lower than in the other study groups.

Analysis of hemodynamic parameters of the examined medical staff in the main and control groups indicates an earlier deterioration of vascular functional status and a potential initial formation of hypertension and atherosclerosis. Decreased activity of the parasympathetic nervous system was observed in the MRI and ultrasound staff based on heart rate variability analysis.

Biochemical and immunological research results did not reveal significant metabolic changes among medical staff in MRI and ultrasound clinics compared to the physiological norm. The heat shock protein (HSP 60) values in the studied group did not differ from those in the control group, indicating no differences in immune reactions between the compared groups and in the structural configuration of molecular enzymes. The functional activity of these enzymes was not reduced (creatine kinase and nitric oxide synthase).

Epidemiological research revealed that the average rate of oncological diseases among ultrasound specialists exceeded that of ophthalmologists by a factor of 2.33. The incidence rate of urogenital diseases among ultrasound specialists was significantly higher, especially in the middle-aged group, compared to the control group. In the pre-retirement age group, musculoskeletal system diseases were more prevalent among ultrasound specialists, significantly surpassing ophthalmologists. During the COVID-19 pandemic in 2020, when comparing the incidence of COVID-19 among the adult population of Kazakhstan with the incidence rate among professional cohorts of medical staff in the Karaganda region (ophthalmologists, specialists in ultrasound and radiology), significant increases were identified, ranging from 7 to 11 times.

The target audience will include scientific researchers in biological laboratories and university departments, developers of modern diagnostic systems, professionals in regional healthcare authorities, and sanitary inspection organizations. Predicting and managing the occupational, deterministic risks of cardiovascular, nervous system diseases, new growths, and reproductive disorders among medical workers will have a significant impact on preventing years of life lost. An information database containing quantitative and qualitative parameters of environmental hazards and predictions of their impact on the health of medical personnel exposed to a complex mix of non-ionizing radiation, noise, vibration, microclimatic, and lighting factors can be provided (on commercial terms) for the creation of extensive databases for further scientific research.

Main publications on the project topic.

1. Ibrayeva L.K., Grebeneva O.V., ShadetovaA.Zh., RybalkinaD.Kh., MinbayevaL.S.,BachevaI.V.,AlekseyevA.V.EffectofNon-ionizingRadiationontheHealthofMedicalStaffof Magnetic Resonance Tomography Offices// Journal of Clinical Medicine of Kazakhstan.-2021.-№18(4). – Р. 16-22.
2. Certificate of Copyright Registration No. 24448 dated March 17, 2022, "Comprehensive Assessment of Detecting the Adverse Effects of a Complex of Non-Ionizing Radiations on the Human Body (A Case Study of Medical Personnel)." Authors: L.K. Ibraeva, B.K. Omarqulov, Z.Z. Zharylqasyn, O.V. Grebeneva, I.V. Bacheva, D.Kh. Rybalkina, A.Z. Shadetova, A.V. Alexeyev, M.V. Rusyaev, Z.B. Sabirov.
3. L.K. Ibraeva Medical and Social Health Problems of Medical Personnel Working in Conditions with Exposure to Non-Ionizing Radiation // International Scientific and Educational Conference "Development and Implementation of Innovative Methods in Education and Science". 11-12.11.2022. Almaty.
4. D. Kh. Rybalkina Morbidity of Medical Personnel with Non-Ionizing Radiation Exposure Factors // International Scientific and Educational Conference "Development and Implementation of Innovative Methods in Education and Science". 11-12.11.2022.Almaty
5. I.V. Bacheva Heart Rate Variability in Medical Personnel of MRI and Ultrasound Departments: Assessment of Physiological Phenomena and Prognostic Significance //International Scientific and Educational Conference "Development and Implementation of Innovative Methods in Education and Science". 11-12.11.2022. Almaty.
6. A.Z. Shadetova, O.V. Grebeneva Assessment of Biological Age of Medical Workers Exposed to Non-Ionizing Radiation // International Scientific and Educational Conference "Development and Implementation of Innovative Methods in Education and Science". 11-12.11.2022. Almaty.
7. Certificate of Copyright Registration No. 37337 dated June 20, 2023, "Methods for Assessing the Impact of a Complex of Non-Ionizing Radiations on Blood Metabolic Parameters." Authors: Z.B. Sabirov, Z.Z. Zharylqasyn, O.V. Grebeneva, L.K. Ibraeva, A.Z. Shadetova, B.K. Omarqulov, A.V. Alexeyev, M.V. Rusyaev, D.Kh. Rybalkina, I.V. Bacheva.
8. Certificate of Copyright Registration No. 37427 dated June 22, 203, "Analysis of Allostatic Load in Medical Personnel Exposed to Non-Ionizing Radiation." Authors: D.Kh. Rybalkina, L.K. Ibraeva, O.V. Grebeneva, I.V. Bacheva, B.K. Omarqulov, Z.Z. Zharylqasyn, A.Z. Shadetova, A.V. Alexeyev, Z.B. Sabirov, M.V. Rusyaev.
9. L.K. Ibraeva, O.V. Grebeneva, B.K. Omarqulov, D.Kh. Rybalkina, Z.Z. Zharylqasyn, I.V. Bacheva, A.Z. Shadetova, A.V. Alexeyev, Z.B. Sabirov, M.V. Rusyaev. Ergonomics of Workstations and Hygienic Safety of Working Conditions for Medical Specialists in Radiological Diagnostic Offices (MRI and Ultrasound) // Methodological Recommendations. Karaganda, 2023. - 31 pages.
10. Z.B. Sabirov, L.K. Ibraeva, A.Z. Shadetova, B.K. Omarqulov, D.Kh. Rybalkina. Cognitive State of Medical Personnel Exposed to a Complex of Non-Ionizing Radiations // X International Scientific and Practical Conference "Modern Strategies and Digital Transformations of Sustainable Development in Society, Education, and Science". 26.06.2023.Moscow.Р. 421-424.
11. A.V. Alexeyev, O.V. Grebeneva, Z.Z. Zharylqasyn, I.V. Bacheva, M.V. Rusyaev. Quality of Life of Medical Personnel Exposed to Non-Ionizing Radiation in Kazakhstan // X International Scientific and Practical Conference "Modern Strategies and Digital Transformations of Sustainable Development in Society, Education, and Science". 26.06.2023.Moscow.Р.415-420.

Research group:

1. Kamyshansky Evgeny Konstantinovich - PhD, project manager. Hirsch index - 2, author ID in Scopus: 56324242300; ORCID http://orcid.org/0000-0001-8975-3612.
2. Tuleubaev Berik Yerkebulanovich - Leading Researcher, Doctor of Medical Sciences, Head of the Department of Surgical Diseases. Hirsch index – 2. Author ID in Scopus: https://www.scopus.com/authid/detail.uri?authorId=57193660354; ORCID https://orcid.org/0000-0002-9640-2463 .
3. Saginova Dina Azimovna – PhD, Head of the Center for Applied Research NSCTO named after acad. N.D. Batpenov, scientific and pedagogical experience of 18 years. Hirsch index –2. Author ID in Scopus:https://www.scopus.com/authid/detail.uri?authorId=57193660757. ; ORCID https://orcid.org/0000-0001-9551-5354 .
4. Kerimbekov Tolegen Ikramovich - Researcher, Assistant of the Department of Surgical Diseases
5. Koshanova Amina Amantaikyzy - Junior researcher, PhD, associate professor of the Department of Surgical Diseases of the NJSC "MUK". Hirsch index – 1. Author ID in Scopus:https://www.scopus.com/authid/detail.uri?authorId=57193661832 ; ORCID https://orcid.org/0000-0001-8620-2196 .
6. Rudenko Alexander Petrovich - Junior researcher, doctoral student of the Department of Surgical Diseases of NAO "MUK".
7. Tashmetov Elyarbek Rozmatzhanovich - Junior researcher doctoral student of the Department of Surgical Diseases of NCJSC "MUK". Author ID in Scopus:https://www.scopus.com/authid/detail.uri?authorId=57211853135 ; ORCID https://orcid.org/0000-0002-2614-4710

In 2023, a histological study was carried out to determine the dynamics of healing of the bone defect. For this purpose, 5 groups (30 rabbits each) were formed: 1 group – allograft with PRP; Group 2 – allograft with bisphosphanate; Group 3 – allograft with BMP-2; 4- allograft with BMP-2+ bisphosphonate; Group 5 – with allograft, which were operated on to form a model of the femoral bone defect. Defects in the groups were filled with various biodegradable materials. The assessment was carried out on days 14 and 30. A previously tested defect of the femur was modeled on rabbits. The results of histological analysis show that the regeneration of the bone defect is more intense with a predominance of newly formed bone tissue in the PRP and BMP-2 groups. In the BMP-2+bisphosphonate and bisphosphonate alone groups, the bone defect was covered predominantly by fibrous tissue.

Information for potential users

Satisfactory results of the pilot study will allow for a clinical trial. In addition, the research results will be useful to a wide range of scientific specialists, undergraduates and doctoral students working in the field of orthopedics, surgery, oncology, etc

Main publications on the project topic

1. Dina Saginova, Elyarbek Tashmetov, Yevgeniy Kamyshanskiy, Marietta Arutyunyan, Ibrahim Rustambek. The histological assessment of new bone formation with zolendronic acid loaded bone allograft in rabbit femoral bone defect// Journal of medicine and life.-2023.-16(4) doi 10.25122/jml-2022-0314. (65 процентиль)
2. Saginova, D.; Tashmetov, E.; Kamyshanskiy, Y.; Tuleubayev, B.; Rimashevskiy, D. Evaluation of Bone Regenerative Capacity in Rabbit Femoral Defect Using Thermally Disinfected Bone Human Femoral Head Combined with Platelet-Rich Plasma, Recombinant Human BoneMorphogenetic Protein 2, and Zoledronic Acid// Biomedicines 2023, 11, 1729. https://doi.org/10.3390/biomedicines11061729 (57 процентиль)
3. Saginova, D.; Tashmetov, E.; Tuleubayev, B.; Kamyshanskiy, Y.; Davanov Sh. Effect of Platelet-rich Plasma Combined with Marburg Bone Bank-prepared Bone Graft in Rabbit Bone Defect Model// Shiraz E-Med J. 2023 September; 24(9):e136960. DOI: https://doi.org/10.5812/semj-136960 (36 процентиль)

Research group

Supervisor– Aliya Ibragimovna Takuadina, Doctor of Philosophy (PhD), Associate Professor of the Department of Informatics and Biostatistics of Karaganda Medical University (Scopus ID: 57219358654, Web of Science Researcher ID: ID AFV-9132-2022, ORCID: 0000-0003-0415-9046).

Performers:

Zhaniya Abaevna Dauletkalieva, Doctor of Philosophy (PhD), Dean of the School of Public Health of Karaganda Medical University (Scopus ID: 58982878100, Web of Science ResearcherID: LVS-3735-2024, ORCID: 0000-0002-4855-2918).

Gaukhar Serikovna Kayupova, PhD, Associate Professor at the School of Public Health of Karaganda Medical University (Scopus ID: 57195056383, Web of Science Researcher ID: LZI-5705-2025 and AFH 1294-2022, ORCID: 0000-0001-9813-2236).

Zhanerke Yerlanovna Bolatova, PhD, Associate Professor, School of Public Health, Karaganda Medical University (Scopus ID: 57254028600, Web of Science Researcher ID: CAF-9520-2022, ORCID: 0000-0002-5624-9943).

Nukeshtaeva Karina Yerlanovna, PhD, Associate Professor at the School of Public Health of Karaganda Medical University (Scopus Author ID: 58100149000, Web of Science Researcher ID: IYS-2376-2023, https://orcid.org/0000-0002-4463-6874 ).

Yerdesov Nurbek Zharkinovich, PhD, Associate Professor at the School of Public Health of Karaganda Medical University (Scopus Author ID: 57547933600, Web of Science Researcher ID: IQW-9082-2023, ORCID: 0000-0001-8095-3140).

Zhamantaev Olzhas Kenzhegalievich, Assistant Professor at the School of Public Health of Karaganda Medical University (http://www.scopus.com/inward/authorDetails.url?authorID=57215498134&partnerID=MN8TOARS , Web of Science Researcher ID: AAW-9071-2020, ORCID: https://orcid.org/0000-0002-9687-2764 ).

Our research has shown that students in Kazakhstan are generally well versed in health issues, but they have certain difficulties and peculiarities:

1. An insufficient level of health literacy was recorded in 4.3% of students of all specialties, and 11.5% have a problematic level of MG. At the same time, these indicators were higher among engineering students compared to others.
2. It is difficult for students to navigate the healthcare system, despite their general understanding of health issues. That is, it can be difficult for our students to understand what to look for, where to go and apply for health problems, as if they are trying to find the right street in an unfamiliar city without a map.
3. Financial problems strongly affect how well people understand health issues. If it is difficult for a person to purchase medicines or undergo an examination, it is more difficult for them to understand medical information.
4. In general, students understand the importance of vaccination well, and those who believe in the benefits of vaccinations are usually better versed in this topic. For example, among the general population, those who understand traffic rules are usually more likely to comply with them, and with vaccination issues, those students who understand "What to get? Where? and When?", these students approach this issue more consciously.
5. Students who have received vaccinations before and those who are older have a better understanding of the issues and importance of vaccination.
6. Even among medical students, one in eight has difficulty understanding the importance of vaccination or obtaining reliable information about vaccinations.
7. The adapted version for assessing health literacy of the HLS19 family has shown its effectiveness.

Information for potential users.

Based on the results obtained, it is possible to further study how students' health literacy changes over time, what contributes to (?) or hinders (?) this. Which teaching methods are most effective for improving health literacy, from what period / level of education? If a clinical and economic analysis is carried out, it is possible to calculate the financial, social and medical benefits of certain preventive interventions aimed at improving MG in the early stages of human life.

Scientific publications within the framework of the project:

Published: 4 articles: 3 of them in the Scopus base list (Q1, Q1, Q2), 1 article in COCA-COLA magazine

1. Kayupova G., Takuadina A., Bolatova Z., Dauletkaliyeva Z., Yerdessov N., Nukeshtayeva K., Zhamantayev O. General, Vaccination, Navigational and Digital Health Literacy of Students Enrolled in Different Medical and Health Educational Programs// Healthcare (Switzerland).- 2024. 12 (9), art. no. 907. DOI: 10.3390/healthcare12090907
2. Nukeshtayeva K., Yerdessov N., Zhamantayev O., Takuadina A., Kayupova G., Dauletkaliyeva Z., Bolatova Z., Davlyatov G., Karabukayeva A. Understanding Students’ Vaccination Literacy and Perception in a Middle-Income Country: Case Study from Kazakhstan Vaccines.- 2024. - 12 (8), art. no. 917. DOI: 10.3390/vaccines12080917
3. Dauletkaliyeva Z, Bolatova Z, Yerdessov N, Nukeshtayeva K, Zhamantayev O, Kayupova G, Takuadina. Health literacy and influencing factors in university students across diverse educational fields in Kazakhstan.// Scientific reports vol. 15,1 3197. 25 Jan. 2025, doi:10.1038/s41598-025-87049-w
4. Zhamantayev, Olzhas & Nukeshtayeva, Karina & Kayupova, Gaukhar & Dauletkaliyeva, Zhaniya & Bolatova, Zhanerke & Takuadina, Aliya & Yerdessov, Nurbek. (2023). Mapping the terrain: A comprehensive exploration of health literacy among youth.// Journal of Clinical Medicine of Kazakhstan. 20. 12-22. 10.23950/jcmk/13917.
5. Report at the 10th AHLA v Hoshimipe International Conference, Etna (May 12-15, 2024) on the topic "Assessment of the level of medical literacy among Kazakhstani students depending on the field of study."

Publications

1. Omarova G., Atikoza Z., Sadirmekova Z, Takuadina A, Abdykeshova D. Devising a methodology for X-ray image contrast enhancement by combining clahe and gamma correction. Eastern-European Journal of Enterprise Technologies, 2022, 3(2-117), p-18-29
2. Aliya Takuadina, Sergey Kabanikhin, Olga Krivorotko, Darya Andornaya and Shuhua Zhang.. Geo-information system of tuberculosis spread based on inversion and prediction. Journal of Inverse and Ill-posed Problems, 000010151520200022, eISSN 1569-3945, ISSN 0928-0219, DOI: https://doi.org/10.1515/jiip-2020-0022 (Q2, 59%). https://www.degruyter.com/document/doi/10.1515/jiip-2020-0022/html
3. Automation of business processes at the enterprise during a brand formation. Ad Alta. 2019;9(1):107-13. Kintonova A, Ye N, Arynova Z, Kussepova L, Karymsakova A, Takuadina A. https://www.magnanimitas.cz/ADALTA/0901/papers/A_kintonova.pdf (Q3)
4. Определение математической модели распространения эпидемии туберкулеза для Казахстана. ВЕСТНИК Алматинского университета энергетики и связи, Алматы, 2019, 1(44), 32-37. Имангалиев Ш.И., Такуадина А.И. https://aues.edu.kz/frontend/web/uploads/vestnik-journal/document/1591159718_nX4cTA.pdf
5. Takuadina A. I., Sagindykov K. M., Kintonova A. Zh. Application of information system of tuberculosis epidemic modeling on the example of Karaganda region. Вестник НИА РК №4 (78) 2020 https://journal.neark.kz/en/primenenie-informaczionnoj-sistemy-modelirovaniya-epidemii-tuberkuleza-na-primere-karagandinskoj-oblasti/
6. Обратные задачи фармакокинетики: анализ и методы решения. ВЕСТНИК ГУ им.Шакарима, серия «Технические науки», №4(84), 2018, 72-76. Такуадина А.И. http://rmebrk.kz/magazine/4787#
7. Идентифицируемость моделей в фармакокинетических исследованиях. ВЕСТНИК Восточно-Казахстанского государственного технического университета им. Д. Серикбаева, серия «Технические науки и технологии», 2018, ТОМ II, 96-101. Такуадина А.И. https://www.ektu.kz/files/vestnik/tech031_2018.pdf

Publications of the core staff of the research group:

1. Tuyen V.Duong, AltynAringazina, Gaukhar Baisunova, et al. Measuring health literacy in Asia: Validation of the HLS-EU-Q47 survey tool in six Asian countries. - J Epidemiol (69%, Q2). 2017 Feb;27(2):80-86. https://doi.org/10.1016/j.je.2016.09.005
2. Kayupova G, Turdaliyeva B, Tulebayev K, Duong TV, Chang PW. Health Literacy among Visitors of District Polyclinics in Almaty, Kazakhstan. - Iran J Public Health (40%, Q3), Vol. 46, No.8, Aug 2017, pp.1062-1070 https://pubmed.ncbi.nlm.nih.gov/28894707/
3. Baisunova G., Darisheva D, Akanov A., Turdaliyeva B., Tulebayev K.,Chang P.W. Health literacy survey in Kazakhstan - The Second International Conference on Health literacy and health promotion. – Taipei, Taiwan. - October 6-8, 2014. P.27-28.
4. Кулов Д.Б, Сергалиев Т.С, Сыздыков М.М, Абдрахманов К.Т, Даулеткалиева Ж.А. Некоторые аспекты осведомленности городского населения о солидарной ответственности за свое здоровье. «Georgian medical news». – 2016. - №1(250). - С. 87 – 92.
5. Даулеткалиева Ж.А., Кулов Д.Б.Результаты изучения мнения медицинских работников о степени ответственности населения за свое здоровье в современных условиях. Астана медициналық журналы. – 2015. - №3.- Б. 139 – 145.
6. Turgambayeva A, Ospanova K, Kissamedenov N, Zhamantayev O, Tashmetov K. Infant Mortality Is One of the Leading Types of Death in the World. Iran J Public Health. 2020;49(3):603-605. doi.org/10.18502/ijph.v49i3.3162
7. Nurbek Yerdessov, Asset Izdenov, Timur Beisenov, Roza Suleimenova, Bakhtiyar Serik, Ermek Sraubaev. Industrial traumatism and occupational morbidity in mining industry of Kazakhstan. J Public Health Res. DOI: 10.4081/jphr.2021.2169.
8. Karina Nukeshtayeva, Marina Lubchenko, Baurzhan Omarkulov, Nailya DeLellis. Validation non-English version of the modified checklist for autism in toddlers-revised with follow-up. J CLIN MED KAZ, Volume 18, Issue 4, pp. 4-11 https://doi.org/10.23950/jcmk/11041
9. Saule Zhakenova, Gaukhar Kayupova, Olzhas Zhamantayev, Zhanerke Bolatova, Zhuldyz Kuanysh. Student’s health literacy level according to university type (preliminary results of the ongoing research in Karaganda, Kazakhstan. - The 7th International Health Literacy Conference. - 10-12.11.2019. – Ho Chi Minh city, Vietnam,
10. Bolatova, Z; Toleubekov, B; Sukhanberdiyev, K ; Tussupova, K. Challenges of Access to WASH in Schools in Low- and Middle-Income Countries: Case Study from Rural Central Kazakhstan. International journal of environmental research and public health, Volume 18, 9852, https://doi.org/10.3390/ijerph18189652.
11. Toleubekov, B; Bolatova, Z; Stafstrom, M. Assessing Access to WASH in Urban Schools during COVID-19 in Kazakhstan: Case Study of Central Kazakhstan. International journal of environmental research and public health, Volume 19, 6438; https://doi.org/10.3390/ijerph19116438.

Research group:

1. Turgunov Y.M. - the head of the project, doctor of medical sciences, Vice-Rector for Scientific and Clinical Work of NJSC MUK; Scopus Author ID - 57215313148, https://orcid.org/0000-0002-6486-3847
2. Shakeyev K. T. - leading researcher of the department of surgical diseases NJSC MUK; Scopus Author ID- 56801885500 https://orcid.org/0000-0002-7802-14644
3. Lavrinenko A.V.- r., master, head of RL of IoLS of NJSC MUK, Scopus Author ID –57193763117, https://orcid.org/0000-0001-9436-8778
4. Avdienko O.V. – junior researcher of RL of IoLS of NJSC MUK; Scopus Author ID -57221804349 https://orcid.org/0000-0001-9122-1279
5. Matyushko D. N. – s.r., PhD, the head of the surgical service of the UC on REM "Multidisciplinary hospital No. 1 of Karaganda" HDKR; Scopus Author ID- 57193721109,https://orcid.org/0000-0002-7884-0507
6. Mugazov M. M. – s.r., PhD, аss. professor of the Department of Emergency Medical Aid, Anesthesiology and Intensive Care; Scopus Author ID – 57209321668, https://orcid.org/0000-0002-7739-8999
7. Аманова Д.Е. – s.r., PhD, аss. professor of the Department of surgical diseases; ResearcherID: AAF-4009-2022, Scopus Author ID 57209749331, https://orcid.org/0000-0001-6293-7833
8. Ogizbayeva A. V. – s.r., PhD, аss. professor of the Department of Emergency Medical Aid, Anesthesiology and Intensive Care, Scopus Author ID – 57331445400, https://orcid.org/0000-0003-1006-1870
9. Asamidanova S.G. – j.r., PhD doctoral student of NJSC MUK. Scopus Author ID 57193911046,https://orcid.org/0000-0002-9819-3079
10. Nuraly Sh.S. – j.r., PhD doctoral student of NJSC MUKhttps://orcid.org/0000-0002-0137-3242
11. Abdrakhman N. – economist NJSC MUK.

for the first time a new model for determining the risk of lethal outcome in patients with multi-organ dysfunction syndrome was developed and implemented based on the established critical APACHE II scores and I-FABP level;

an online calculator for predicting lethal outcome in hospitalized patients named as LOP MODS was developed: speccters.github.io ;

all stages of research and development were realized according to the calendar plan;

the results of the scientific work were presented at the international conferencea ISW 2024, ISICEM 2025, Euroanaesthesia 2025;

1 review published in a peer-reviewed domestic publication recommended by SHEQAC;

4 article published in a peer-reviewed scientific publications with a percentile of CiteScore in the Scopus database more than 35%;

received 2 certificates of entry of information into the state register of rights to objects protected by copyright, the developed methodology was implemented in the work of 4 hospitals in the city of Karaganda.

Applicability of the obtained scientific results: methods for determining the studied biomarkers, as well as measuring intra-abdominal pressure, their relationship will allow further research in the study of intestinal microflora translocation not only in patients with multiple organ dysfunction. Improving the image of the Collective Use laboratory will help attract investments and conduct research by research teams in Kazakhstan and foreign partners. Mastering modern high-tech research methods will create the prospect of commercialization in the form of creating a learning platform (master classes, advanced training, specialization in the workplace, etc.).

Target consumers of the results: researchers, physicians; patients with multiple organ dysfunction syndrome of various genesis.

1. The results of the scientific work were presented at the international conferences:

International Surgical Week ISW 2024, Kuala Lumpur, Malaysia 25-29 August 2024 in the section «Poster Exhibition presentation»:

Ogizbayeva A.V., Turgunov Ye.M., Avdiyenko O.V., Amanova D.Ye., Asamidanova S.G. The biomarkers of bacterial translocation and intestinal wall damage in the patients with multiple organ dysfunction syndrome. - PE329. - Abstract Volume ISW 2024. - P. 701.

44th International Symposium on Intensive Care & Emergency Medicine (ISICEM 2025), March 18-21, 2025, Brussels, Belgium, в секции «Poster presentation»:

A Ogizbayeva, Y Turgunov, S Assamidanova, O Avdiyenko, D Matyushko. Intestinal fatty acid binding protein and lipopolysaccharide-binding protein in surgical patients with multiple organ dysfunction syndrome // 44th International Symposium on Intensive Care & Emergency Medicine // Critical Care. - 2025. - V. 29 (Suppl 1). - N. 97 (2025). - P032. doi.org

Euroanaesthesia 2025, May 25 - 27, 2025, Lisbon, Portugal, в постерной секции «Intensive Care Medicine»:

A. Ogizbayeva, S. Assamidanova, Y. Turgunov. Biomarkers of bacterial translocation and intestinal wall damage in patients with multiple organ dysfunction syndrome // European Journal of Anaesthesiology. - June 2025. - Volume 42. - e-Supplement 63. - 51AP01-2. doi.org

2. 4 article published in a peer-reviewed scientific publication with a percentile of CiteScore in the Scopus database more than 35%:

Turgunov YeM, Ogizbayeva AV, Mugazov MM, Asamidanova SG. Biomarkers of intestinal wall damage in multiple organ dysfunction syndrome. Russian Journal of Anaesthesiology and Reanimatology (Anesteziologiya i Reanimatologiya). 2024;2:114–120. (In Russ.).doi.org

Russian Journal of Anaesthesiology and Reanimatology (Anesteziologiya i Reanimatologiya) – Scopus 45% (Q3), ISSN:0201-7563

Turgunov, Yermek, Ogizbayeva, Alina, Asamidanova, Sofiko, Avdiyenko, Olga, Amanova, Dana, Aukenov, Nurlan, Mugazov, Miras, Biomarkers of Bacterial Translocation and Intestinal Wall Damage in Patients With Multiple Organ Dysfunction Syndrome, International Journal of Clinical Practice, 2024, 3015526, 13 pages, 2024. doi.org

International Journal of Clinical Practice – Scopus 85%, (Q1), Web of Science по JCR Q2, ISSN:1742-1241

Turgunov, Y.; Ogizbayeva, A.; Assamidanova, S.; Matyushko, D.; Mugazov, M.; Amanova, D.; Nuraly, S.; Sharapatov, Y. The Role of I-FABP, REG3α, sCD14-ST, and LBP as Indicators of GI Tract Injury in MODS Patients. Diagnostics. 2025, 15, 515.doi.org

Diagnostics – Scopus 62% (Q2), Web of Science по JCR Q1, ISSN: 2075-4418.

Turgunov Ye.M., Ogizbayeva A.V., Assamidanova S.G., Avdiyenko O.V., Matyushko D.N., Izdenov A.K., Kaliyeva D.K., Shakeyev K.T. I-FABP and LBP as predictive markers for outcomes in surgical patients with multiple organ dysfunction: a prospective observational cohort study// Annals of Critical Care. – 2025. - V.4. – Р.126–136. doi.org

Annals of Critical Care – Scopus 53% (Q2), ISSN:1726-9806.

3. 1 review published in a peer-reviewed domestic publication recommended by SHEQAC:

Asamidanova S.G., Ogizbayeva A.V., Gritskova A.Yu., Turgunov Ye.M. Interrelation of intraabdominal hypertension and markers of gastrointestinal tract injury in patients with multiorgan dysfunction // Nauka i Zdravookhranenie [Science & Healthcare]. 2024, (Vol.26) 1, pp. 168-178. doi.org

4. The monograph has been published in print. The monograph has been approved and permitted for publication by the Senate of the Karaganda Medical University (protocol No. 10 dated May 29, 2025).

Огизбаева А.В., Тургунов Е.М., Асамиданова С.Г. Биомаркеры бактериальной транслокации и повреждения кишечной стенки в прогнозировании исходов при синдроме мультиорганной дисфункции // Монография. - ТОО «Типография АРКО», 2025. – 87 с.

5. Certificates of entry of information into the state register of rights to objects protected by copyright:

Огизбаева Алина Виталиевна, Тургунов Ермек Мейрамович, Аманова Дана Ерлановна, Мугазов Мирас Мугазович, Матюшко Дмитрий Николаевич, Асамиданова Софико Гиевна. Математическая модель прогнозирования летального исхода у пациентов с синдромом мультиорганной дисфункции. № 53726 от «22» января 2025 года

Асамиданова Софико Гиевна, Огизбаева Алина Виталиевна, Кожина Диана Александровна, Тургунов Ермек Мейрамович. Онлайн-калькулятор «Lethal outcome prediction in multiple organ dysfunction syndrome (LOP MODS)». № 56240 от «1» апреля 2025 года

Research Group

Yukhnevich Y.A., Lavrinenko A.V., Kadyrova I.A., Solomadin M.V., Simokhina N.A., Ryazanets D.A., Turemuratova A.B., Seisenbekova A.K., Shkreba A.P., Otemis A.

The prevalence of H. pylori was 29.81% (95% CI: 25.37–34.67%) in Karaganda. The study identified the following polymorphisms in the vacA genes (s1/s2, s1a, s1b, s1c, m1/m2), as well as the CagE, CagA, IceA1, IceA2, and BabA2 genes.

The most common H. pylori strains were BabA2 (63.63%), vacA (62.72%), CagE (39.09%), IceA2 (35.45%), and CagA (30.90%).

Expression of the vacA s1/s2 gene is closely associated with chronic gastritis (p=0.008), as is the VacAs1a polymorphism (p=0.044). vacA s1/s2 strains were significantly associated with gastric ulcer cases (P=0.002), while VacAs1a strains were also associated with gastric ulcer disease (P=0.014). The presence of H. pylori S_23 strains was significantly associated with chronic gastritis in patients (P<0.001). The AMP S_23 gene was also significantly associated with gastric ulcer disease (P<0.001) and duodenal ulcer disease (P=0.039), indicating a possible role for this gene in the development of these conditions. Further research is required.

According to the results of susceptibility testing, resistance to antimicrobial agents was 20.58%, 20.98%, 9.8%, 4.9%, 9.8%, and 9.8%. Resistance to AMX was 20.98%, complicating empirical eradication therapy, as AMX is found in most combination therapies recommended by the Maastricht Convention No. 6.

The main mutation in CLA-resistant strains was the 23S rRNA resistance gene A2144G. Significant associations were found between IceA1 and antral localization of endoscopic lesions (p < 0.01), suggesting its localized role in disease pathogenesis. The presence of the VacA m1/m2 polymorphism was associated with damage to the pyloric mucosa. A significant positive correlation was observed between lesions in the corpus of the stomach and the presence of the CagA gene (p < 0.05).

A more detailed study demonstrated the comparability of antibiotic resistance determination using a classical microbiological test with the identification of the target associated with antibiotic resistance in H. pylori using a molecular genetic method. The association between the presence of PBP1 (χ² - 53.322440) and antibiotic resistance to amoxicillin is statistically significant (p - <0.001, Cramer's V coefficient - 0.518946). The correlation between the presence of PBP1 (χ2 - 33.915000) and resistance to nitrofurantoin is also statistically significant (p - 0.002122, Cramer's V coefficient - 0.413869). The association between the presence of PBP1 (χ2 - 38.115000) and their resistance to tetracycline is also statistically significant (p <0.001, Cramer's V coefficient - 0.438748), as well as levofloxacin (χ2 - 33.078065, Cramer's V coefficient - 0.408731, p - 0.002807), clarithromycin (χ2 - 63.596591 0, Cramer's V coefficient - 0.112927, p - 0.001556) and metronidazole (χ2 -38.457143, Cramer's V coefficient - 0.440713, p <0.001).

A link was found between gastric ulcers and the consumption of tap water (3.8) and boiled water (4.0), as well as between duodenal ulcers and the consumption of well water (2.7) and tap water (2.4). Furthermore, working as a laborer was associated with an increased risk of developing duodenal ulcers (2.6).

A significant association was found between the presence of the Ice A2 gene in the pathogen and a positive DUT result after eradication treatment (p=0.064). Patients with the Ice A2 genotype have a 2.429-fold increased risk of treatment failure (OR) at p=0.005.

Information for potential users.

This analysis demonstrates the inadequacy of current epidemiological studies and highlights the importance of further research in this area. This study aims to determine the prevalence of H. pylori, understand the mechanisms of antibiotic resistance, and identify predictors of eradication therapy failure. Understanding local epidemiology can help optimize treatment strategies and improve outcomes for patients with H. pylori-related diseases in Karaganda, Kazakhstan.

Two articles have been published in peer-reviewed scientific journals with a CiteScore percentile in the Scopus database of at least 35 (thirty-five):

1. Alyona L, Aizhan S, Aidana T, Alexey S, Yekaterina Y. Review of Methods for Detection Helicobacter pyloriin Kazakhstan// JGH Open. – 2025. - Jan 17;9(1):e70101. doi.org

JGH Open – Scopus 50% (Q2), ISSN:2397-9070.

2. Seisenbekova A, Laryushina Y, Yukhnevich Y, Lavrinenko A, Shkreba A. Prevalence and riskfactorsof H. pylori infection among outpatient inKaragandacity (Kazakhstan) // Future Sci OA. - 2025 Dec;11(1):2461429. Epub 2025 Feb 10. doi.org

Future Sci OA – Scopus 80% (Q1), ISSN:2056-5623.

One article was published in the peer-reviewed scientific journal of the КОКСНВО:

Aizhan Seisenbekova, Alyona Lavrinenko, Yelena Laryushina, AyatbekOtemis, Olga Volnovaya, Maxim Solomadin, YekaterinaYukhnevich, Antonella Chesca. Antibiotic Resistance of Helicobacter pylori// Data from Central Asia J CLIN MED KAZ. – 2025. – V/22(4). - Р.62-68. doi.org

Research Group

1. Akhmetova Saule Baltabaevna – Project manager, Head of the Department of Clinical Immunology, Allergology and Microbiology of NCJSC «KMU». The Hirsch index is – 6,https://www.scopus.com/authid/detail.uri?authorId=25030129100,https://orcid.org/0000-0002-8112-742X
2. Tuleubaev Berik Yerkebulanovich – a leading researcher, MD, Professor, Head of the Department of "Surgical Diseases" of NCJSC «KMU». The Hirsch index is – 2, https://www.scopus.com/authid/detail.uri?authorId=57193660354,https://orcid.org/0000-0001-9551-5354
3. Koshanova Amina Amantaykyzy – researcher, Associate professor of the Department of Surgical Diseases of the NCJSC «KMU». The Hirsch index is – 1,https://www.scopus.com/authid/detail.uri?authorId=57193661832, https://orcid.org/0000-0001-8620-2196
4. Belyaev Ilya Andreevich – researcher, Assistant of the Department of Clinical Immunology, Allergology and Microbiology of NCJSC «KMU».https://www.scopus.com/authid/detail.uri?authorId=55935731100, https://orcid.org/0000-0003-3373-2059
5. Kosilova Ekaterina Yurievna – junior researcher, a 3rd-year doctoral student of the Department of Surgical Diseases of the NCJSC «KMU».
6. Amirkhanova Zhanerke Talgatkyzy – junior researcher, assistant of the Department of Clinical Immunology, Allergology and Microbiology of NCJSC «KMU».

Evaluation of X-ray changes during filling of bone defects with biocomposite in the studied groups in comparison with hemopollection

All experimental animals underwent X-ray examination on the 14th, 30th and 60th days after surgery. The examination was performed under general anesthesia (zoletil 0.1 mg/kg, Virbac, USA) in the Veterinary Clinic of Traumatology and Orthopedics of Dr. Kryukov. Radiography was performed in two standard projections — straight and lateral.

X-ray assessment of bone defect healing

To quantify the dynamics of reparative osteogenesis, the Lane–Sandhu X-ray scale (Lane & Sandhu, 1987) was used, which is widely used and validated in experimental models of bone defect healing in small laboratory animals, including rats and mice (Tawonsawatruk et al., J Orthop Res, 2014; J Orthop Surg Res, 2025).

Evaluation It was performed according to three key radiological criteria reflecting the successive stages of bone regeneration:

1. bone marrow formation;
2. severity of the defect line;
3. The degree of bone remodeling.

Each parameter was evaluated on a four—point scale (0-4 points), where 0 corresponded to the absence of signs of regeneration, and 4 - to the complete restoration of the anatomical structure and radiological bone density. The maximum total score was 12 points and corresponded to the completed X-ray healing of the defect.

Principles of evaluation and interpretation of results

The X-ray images were analyzed in a blind mode by two independent experts. For each animal, at each observation period (14, 30, and 60 days), scores were determined for all three criteria, followed by the calculation of the total score. The average values obtained were used for statistical comparison between the groups.

Interpretation of the total score:

• 0-3 points — absence of radiological signs of healing;
• 4-7 points — partial fusion, callus formation, initial mineralization;
• 8-10 points — pronounced osteogenesis with signs of remodeling;
• 11-12 points — complete X-ray healing of the defect.

X-ray examination results

On the 14th day, signs of an early phase of repair were revealed in all the studied groups: clear contours of the bone edges of the defect were preserved, weakly expressed shadows of fibrous-osteoid tissue were visualized. At the same time, the most pronounced formation of periosteal calluses was noted in the MicroNOC group, which was reflected in slightly higher total scores on the Lane–Sandhu scale.

By day 30, partial filling of the defect with osteoid tissue and a moderate decrease in the clarity of the defect line were observed in the Hemoplomb and MacroNSC groups. At the same time, in the MicroNSC group, the defect line was practically not visualized in most cases, which indicated the formation of mature bone marrow and more active mineralization of the regenerate.

On the 60th day, a continuous bone structure with signs of pronounced remodeling and restoration of the cortical plate was formed in animals of the MICRONC group. In the Hemoplomb group, the reparative processes were moderate, whereas in the MacroNSC group, healing was slow and characterized by areas of heterogeneous radiological density.

Figure 1. A. On day 30, the defect was closed by 50% in the hemopollection group, B. by less than 30% in the Macro NSC group, by 100% in the Micro NSC group; C. on day 60 in the hemopollection group and in the Micro NSC group, the defect was eliminated, closed by 100%, in the Macro NSC group – by 50%.

Statistical analysis

There were no statistically significant differences between the groups on day 14 (P₁, p₂, p₃ > 0.05), reflecting the similarity of the initial inflammatory-reparative phase of osteogenesis. On the 30th day, the indices of the MicroNNC group significantly exceeded the values of the Hemoplomb and MacroNNC groups (P₂, p₃ < 0.05). On day 60, statistically significant differences remained between microns and macrons (p < 0.01), as well as between microns and Hemoplomb (p < 0.05), which indicates a more pronounced consolidation and remodeling of bone when using a microdispersed nanocellulose composite.

The X-ray data obtained are consistent with the results of the histological examination. The use of MICRONSCS was accompanied by more intensive bone marrow formation and subsequent remodeling, which indicates a pronounced osteoconductive and osteoinductive activity of the biocomposite during intramedullary localization. In the Hemoplomb group, repair proceeded according to the type of physiological bone healing with moderate osteogenesis activity. In the MacroNSC group, the slowdown in osteoreparative processes is probably due to a less uniform distribution of the material and a reduced degree of cellular infiltration.

Based on the data obtained in vivo, forecasts of further application in clinical practice, in particular traumatology, transplantology, neurosurgery, will be given.

1 article and/or review has been published in a peer-reviewed scientific publication indexed in the Science Citation Index Expanded of the Web of Science database and/or having a CiteScore percentile in the Scopus database of at least 35 (thirty-five).

Article title: Tyleubaev, B.,Kamyshanskiy, Y., Saginova, D., Ahmetova,S., Vinokurov, V., Koshanova, A. et al. (2024)Comparative histomorphological assessmentof the osteoinductive capacity of ananofibrillated cellulose‐based composite andautologous blood clot. Journal of Experimental Orthopaedics, 11, e70067.https://doi.org/10.1002/jeo2.7006714 of 14 |

Submitted articles: In Vivo 52 percentile in the Scopus database:

1. Tyleubaev B., Kamyshanskiy Y., Saginova D., Ahmetova S., Vinokurov V., Koshanova A., Kossilova Y. Osteoregenerative Potential and Remodeling of a Composite Based on Nanofibrillated Cellulose, Xenograft, and Phenol-Formaldehyde Resin.
2. Tyleubaev B., Kamyshanskiy Y., Saginova D., Ahmetova S., VinokurovV., Koshanova A., Kossilova Y. A Histological Study Under Normal and Infected Bone Wound Conditions.

Published 1 article by KOKNVO: Тулеубаев Б.Е., Косилова Е.Ю.,ҚошановаА.А., Авромиди И.К. «Наноматериалдарды дамытудың заманауи тенденцияларымен оларды медицинада қолдану» Traumatology and Orthopedics of Kazakhstan, Spetial issue. Number 76 (2025). DOI: https://doi.org/10.52889/

Speaking at international conferences:

Presentation of a poster presentation: 26th EFORT Congress 11 – 13 June 2025 in Lyon, France: Evaluation Of A New Nanocellulose-Based Biocomposite, Including Microbiological, X-Ray And Histomorphometric Control In Vivo.

Presentation of a poster presentation: Мадрид Испания: 45 th SICOT Orthopaedic World Congress Madrid Spain 3-5 september 2025: Evaluation Of A New Nanocellulose-Based Biocomposite, Including Microbiological, X-Ray And Histomorphometric Control In Vivo.

Received the author's right: KOSILOVA EKATERINA YURYEVNA, Tuleubaev Berik Yerkebulanovich, Koshanova Amina Amantaikyzy, Saginova Dina Azimovna, Kamyshansky Evgeny Konstantinovich, Darybaev Daryn Maratuly, Avromidi Ivan Konstantinovich "On entry into the State Register of rights to copyrighted objects" No. 54701 dated February 17, 2025.

Type of copyright object: a work of science.

Object name: A technique for creating an optimal model of a tubular bone defect and filling it with a biocomposite in vivo. 12.02.2025

Research group:

Kishkentayeva Anarkul S., ORCID 0000-0002-9169-3492. Scopus author ID 55799125700.

1. Mukhametzhanova G., Asanova G., Adekenova G.S., Kishkentayeva A., Adekenov S. Chartolepis intermedia Boiss. and Centaurea ruthenica Lam. – new medicina plants containing pharmacologically active compounds //Open Access Macedonian Journal of Medical Sciences.- 2022.-№10.-P.56–64 (Импакт-фактор 1.117, процентиль по CiteScore 1.3, Q3), doi.org
2. Adekenov S.M., Kishkentayeva A.S., Khasenova A.B., Gatilov Y.V., Bagryanskaya I.Y.New arylhalo-derivatives of grosshemin //Chemistry of Natural Compounds.- 2021.-57(4).-P. 685–690 (Импакт-фактор 0.708, процентиль по CiteScore 1.3, Q3), doi.org.
3. Адекенов С.М., Кишкентаева А.С., Шаймерденова Ж.Р., Атажанова Г.А. Бимолекулярные соединения на основе природных метаболитов //Химия природных соединений. - 2018.-№3-Р.394-399 (Импакт-фактор 0.708, процентиль по CiteScore 1.3, Q3), doi.org.
4. Schepetkin I.A., Kirpotina L.N., Mitchell P.T., Kishkentaeva А.S., Shaimerdenova Zh.R., Atazhanova G.A., Adekenov S.M., Quinn M.T. The natural sesquiterpene lactones arglabin, grosheimin, agracin, parthenolide, and estafiatin inhibit T cell receptor (TCR) activation// Phytochemistry. - 2018.-V. 146.-P. 36-46 (Импакт-фактор 4,004, процентиль по CiteScore 6.2), doi.org.
5. Kishkentayeva A.S., Adekenov S.M., Drašar P.B. Production technologies of pharmacologically active sesquiterpene lactones //Eurasian chemico-technological journal.-2018.-№4.-P.289-297 (Импакт-фактор 0.157, процентиль по CiteScore–1.3, doi.org).

Shulgau Zarina T., Researcher ID L-1400-2015, ORCID 0000-0001-8148-0816, Scopus author ID 25651954000.

1. Stalinskaya A.L., Shulgau Z.T., Sergazy Sh.D., Gulyaev A.E., Turdybekov D.M., Turdybekov K.M., Kulakov I.V. Synthesis, structure, and biological activity of N-p-(dimethylamino)-N′-(p-dimethylaminobenzylidene)-N,N′′-diphenylbenzohydrazonohydrazide // Russian Journal of General Chemistry. – 2022. –V. 92, No2. – P. 147–153 (Импакт-фактор0.868, процентиль по CiteScore 1.3, Q4), doi.org.
2. Sergazy Sh., Gulyayev A., Amangeldiyeva A., Nurgozhina A., Nurgaziyev M., Shulgau Z., Chulenbayeva L., KhassenbekovaZh., Kushugulova A., Aljofan M. Antiradical and cytoprotective properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats // Front. Pharmacol. - 2021.-V.12 (Импакт-фактор5.33, процентильпоCiteScore – 6,2Q1), doi.org.
3. Sergazy S., Shulgau Z., Fedotovskikh G., Chulenbayeva L., Nurgozhina A., Nurgaziyev M., Krivyh E., Kamyshanskiy Ye., Kushugulova A., Gulyayev A., Aljofan M. Cardioprotective effect of grape polyphenol extract against doxorubicin inducedcardiotoxicity // Scientific Reports. - 2020. – V.10, No 1. – P. 1-12 (Импакт-фактор 4,576, процентиль по 7.1, Q1), doi.org.
4. Kayukova L.A., Uzakova А.B., Baitursunova G.P., Dusembaeva G.Т., Shulgau Z.Т., Gulyayev A.Ye., Sergazy Sh.D. Inhibition of α-amylase and α-glucosidase by new -aminopropionamidoxime derivatives // Pharmaceutical Chemistry Journal. – 2019. - Vol. 53, No. 2. – P. 129-133 (Импакт-фактор 0.837, процентиль по CiteScore – 6.9, Q4), doi.org.
5. Shulgau Z., Sergazy Sh., Krivoruchko T., Kenzhebayeva N., Sagindykova B., Gulyayev A. Osteoprotective properties of RNA-containing drug osteochondrin S on the model of insufficiency of sex hormones in rats // Int. J. Morphol. – 2017. – Vol. 35 (4). – P. 1233-1238 (Импакт-фактор 0.499, процентиль по CiteScore – 0.7, Q3), doi.org.
6. Sergazy Sh., Shulgau Z., Zhulikeyeva A., Ramankulov Y., Palamarchuk I.V., Kulakov I.V. Cytoprotective activity of newly synthesized 3-(arylmethylamino)-6-methyl-4-phenylpyridin-2(1H)-ones derivatives// Molecules. – 2022. – V.27(17). - 5362 (Импакт-фактор 4.927, процентиль по CiteScore – 5.9, Q1), doi.org.
7. Keyer V., Syzdykova L., Zauatbayeva G., Zhulikeyeva A., Ramanculov Y., Shustov A.V., Shulgau Z. Tilorone and cridanimod protect mice and show antiviral activity in rats despite absence of the interferon-inducing effect in rats//Pharmaceuticals. – 2022. – V.15, No5. – P. 617 (Импакт-фактор 5.215, процентиль по CiteScore – 4.0, Q2), doi.org.

Shakarimova Kuanysh K., ORCID 0000-0003-0336-7142.

Shaimerdenova Zhanar R., ORCID 0000-0003-4910-3695. Scopus Author ID: 57194650829

1. Адекенов С.М., Кишкентаева А.С., Шаймерденова Ж.Р., Атажанова Г.А. Бимолекулярные соединения на основе природных метаболитов //Химия природных соединений. - 2018.-№3-Р.394-399 (Импакт-фактор 0.708, процентиль по CiteScore 1.3, Q3), doi.org.
2. Schepetkin I.A., Kirpotina L.N., Mitchell P.T., Kishkentaeva А.S., Shaimerdenova Zh.R., Atazhanova G.A., Adekenov S.M., Quinn M.T. The natural sesquiterpene lactones arglabin, grosheimin, agracin, parthenolide, and estafiatin inhibit T cell receptor (TCR) activation// Phytochemistry. - 2018.-V. 146.-P. 36-46 (Импакт-фактор 4,004, процентиль по CiteScore 6.2), doi.org.
3. Khlebnikov A.I., Schepetkin I.A., Kishkentaeva A.S., Shaimerdenova Zh.R., Atazhanova G.A., Adekenov S.M., Kirpotina L.N., Quinn M.T. Inhibition of T cell receptor activation by semi-synthetic sesquiterpene lactone derivatives and molecular modeling of their interaction with glutathione and tyrosine kinase ZAP-70 // Molecules. – 2019. – 24(2). – Р. 350-367 (Импакт-фактор 4.927, процентиль по CiteScore – 5.9, Q1), doi.org.

Adekenova Aigerim S., ORSID 0000-0003-1378-0985, Scopus author ID 55596615800.

1. S.M. Adekenov, D.L. Savchenko, A.O. Kenzhebekov, A.N. Zhabaeva, A.S. Adekenova, V.V. Polyakov Development of therapeutic and cosmetic cream based on flavonoids//Fitoterapia.-Vol. 180.- 2025.- 106283. doi.org.
2. S. M. Adekenov, Zh. R. Shaimerdenova, D.K. Nurkadirov, A.S. Adekenova, A. Berthod Purification and Chromatographic Analyses of Cyclopentadienone Guaianolides from Artemisia leucodes Schrenk //Chromatographia.- 2024.-Vol. 87.- P. 375–383.

During the reporting period, new triazole- and bis-triazole-containing derivatives of coumarins were obtained. Optimal conditions for their chemical modification were selected. The structures of the synthesized compounds were established using modern methods: ¹H and ¹³C NMR spectroscopy, IR and UV spectroscopy, and mass spectrometry. A bioscreening of the synthesized compounds was carried out. In vitro experiments were performed to study their biological activities. These included the antiradical, hemorheological, antimicrobial, cytotoxic, antidiabetic, hemagglutinating, neuraminidase, infectious and virus-inhibiting activities. Cytotoxic effects were also evaluated on human cancer cell lines.

The developed synthesis methods based on coumarins will be used in research institutes to modify natural compounds.

Four scientific papers were published based on the research results. Three of them appeared in journals indexed in Scopus and Web of Science. One paper was published in a journal recommended by the Committee for quality assurance in the sphere of science and higher education. One utility model patent was obtained. In addition, one patent application was submitted to the Patent Office of the Republic of Kazakhstan.

1. Kishkentayeva A. S., Shaimerdenova Zh. R., Kairatova Zh. S., Akhmetova S. B. 2-Oxo-2H-chromen-3-carbonyl chloride with antimicrobial activity. Republic of Kazakhstan Utility Model Patent No. 10368, filed and issued November 21, 2024
2. Kishkentayeva A. S., Shaimerdenova Zh. R., Telmenbetova A. B., Shakarimova, K. K. Determination of the structure of a dimeric compound based on the coumarin umbelliferone. Bulletin of Toraighyrov University, Series: Physics, Mathematics and Computer Science, № 4, 2024.- P.161-169. DOI: 10.48081/XYER6975.
3. Kishkentayeva A., Kopbalina K., Shaimerdenova Z., Shults E., Gatilov Y., Pankin D., Smirnov M., Povolotckaia A., Turdybekov D., Mazhenov N. Investigation of N-(2-oxo-2H-chromen-3-carbonyl)cytisine’s crystal structure and optical properties// Materials. – 2025. – V.18(3). - 3153. doi.org (процентиль 79, Q1).
4. Kishkentayeva A.S, Hamad M.S, Pokrovsky M.A., Shaimerdenova Z.R., Adekenova A.S., Mambeterzina G.K., Savelyev V.A., Pokrovsky A.G., Shults E.E. Synthesis and biological evaluation of some coumarin–triazole conjugates as potential anticancer agents// Sci. Pharm. – 2025. – V. 93(2). - 16 doi.org (процентиль 59, Q2).
5. K.Kopbalina, A.Adekenova, Zh.Shaimerdenova, Zh. Kairatova, K. Shakarimova, D.Pankin, M.Smirnov, A.Kishkentayeva, M.Artykbayeva, R.Jalmakhanbetova. Investigation of N-(2-oxo-2H-chromen-3-carbonyl) cytisine’s Molecular Structure in solution// Molecules – 2025. –Vol.30(20), 4139 (Web of Science Q2, Scopus, 82%).

1. Kadyrova I.A. - PhD in the specialty "Medicine", associate professor, doctor of clinical and laboratory diagnostics, senior researcher of the Research laboratory of NJSC «KMU». h-index - 5, ORCIDID 0000-0001-7173-3138.
2. Babenko D.B. - PhD in the specialty "Medicine", specialist in molecular genetics research methods, bioinformatics, statistical analysis and machine learning methods. h-index - 5 (Scopus); 2 (Web of Knowledge), Author ID Scopus: 55935641200 SPIN-код: 3567-2768, Researcher ID P-8052-2017, ORCID 0000-0003-2280-2146.
3. Egorov Sergey - PhD in "Immunology" (University of Toronto). In the project, he acts as a consultant on immunological methods of research and interpretation of immunopathogenesis. h-index – 17, ORCID ID 0000-0002-7136-7921.
4. Lavrinenko A.V. - Master in the specialty "Medicine", doctoral student of 3 years of study, researcher of the Research laboratory of NJSC "KMU" of NJSC «KMU». h-index - 1, ORCID 0000-0001-9436-8778.
5. Kolesnichenko S.I. - Junior Researcher of the Research laboratory of NJSC «KMU» . ORCID 0000-0003-3515-8900.
6. Stupina E.A. - Head of the department of drip and neuroinfections, infectious disease doctor of the regional clinical hospital in Karaganda.
7. Sultanbekova A.A. - otolaryngologist, PhD doctoral student.

The project implementation has been fully completed.

The research team evaluated the performance of the insilico machine learning algorithm on combined data from South America and Kazakhstan and also trained their own ML models with the aim of differentiating samples with SARS-CoV-2. The SVM-R and DT models showed especially high performance, surpassing other models, with ROCAUC scores of 0.983 and 0.972, respectively. SVM-R is particularly effective in distinguishing samples from Kazakhstan, achieving an accuracy of 88.0% for SARS-CoV-2+, 95.0% for NCARI, and 78.0% for AC. The potential of MALDI MS as an innovative diagnostic tool for identifying various respiratory infections is emphasized, as well as the need for further optimization and validation of the MALDI MS/ML method before its integration into routine clinical practice, considering the changing epidemiological situation.

The areas of application of the research results include: infectious diseases, clinical microbiology, laboratory diagnostics.

2021 year:
1. Lavrinenko A., Kolesnichenko S., Turmukhambetova A., Kadyrova I. Respiratory pathogens co-infection in patients with COVID-19 pneumonia in Kazakhstan//Microbiology society. Annual conference online 2021. Poster abstract book 26–30 April 2021, Р 282.
2022 year:
2. Kadyrova I, Yegorov S, Negmetzhanov B, Kolesnikova Y, Kolesnichenko S, Korshukov I, et al. (2022) High SARS-CoV-2 seroprevalence in Karaganda, Kazakhstan before the launch of COVID-19 vaccination. PLoS ONE 17(7): e0272008. https://doi.org/10.1371/journal. pone.0272008 (Scopus -87 процентиль, Q1).
3. Kadyrova I.A., Sultanbekova A.A., Barkhanskaya V.I., Kolesnichenko S.I., Kolesnikova E.А., Lavrynenko А.V., Korshukov I.V., Bekov Ye.K., Yegorov S.V., Babenko D.B. Application of MALDI MS mass spectrometry for the detection of SARS-CoV-2 in nasopharyngeal swabs//Systematic review Science & Healthcare, 2022. (Vol. 24) 5, Р. 36-44. (КОКСОН).
4. I. Kadyrova, S. Kolesnichenko, Ye. Kolesnikova, I. Korshukov, V. Barkhanskaya, A. Sultanbekova, D. Babenko SARS-COV-2 Detection in MALDI-TOF Mass Spectra by Machine Learning/ Int'l Conference on Mathematical Modeling in Physical Sciences September 5-8, 2022 Virtual, on-line Conference
5. S.I. Kolesnichenko, I.A. Kadyrova, I.V. Korshukov, Ye.A. Kolesnikova, V.I. Barkhanskaya, D.B. Babenko SARS-COV-2 DETECTION USING MALDI-TOF AND MACHINE LEARNING APPROACH// IX International Conference of Young Scientists: Virologists, Biotechnologists, Biophysicists, Molecular Biologists and Bioinformaticians within the framework of the OPENBIO Open Communications Platform.
2023 year:
6. Yegorov S., Kadyrova I. et al «Application of MALDI MS and Machine Learning to Detection of SARS-CoV-2 and non-SARS-CoV-2 Respiratory Infections» находится на рецензировании в журнале PLOS One, опубликован препринт https://doi.org/10.1101/2023.08.31.23294891
7. А.А. Султанбекова, И.А. Кадырова, Д.Б. Бабенко. Maldi-Tof масс-спектрометрия жәнемашиналық оқыту әдістерін қолдануарқылы sars-cov-2 вирусындиагностикалаудың экспресс әдісін бағалау// Фармакология Казахстана, 2023, № 1, С. 262-276
8. А.А. Султанбекова. Опыт применения время пролетной масс-спектрометрии (MALDI-TOF) для детекции вирусных патогенов, в частности короновируса. Систематический обзор Фармакология Казахстана, 2023, № 4, С. 97-106.
9. Kadyrova, I., Kolesnichenko, S., Korshukov, I., Kolesnikova, Y., Barkhanskaya, V., Lavrinenko, A., Babenko, D. (2023). SG-APSIC1053: Detection of SARS-COV-2 in nasopharyngeal swags with MALDI-TOF MS and machine learning. Antimicrobial Stewardship & Healthcare Epidemiology, 3(S1), S2-S3. doi:10.1017/ash.2023.9
10. Dmitriy Babenko et al Application of MALDI MS to Differentiate SARSCoV-2 and Non-SARS-CoV-2 Symptomatic Infections in the Early and Late Phases of the Pandemic// Conference Proceedings, Zurich Switzerland July 24-25, 2023.

Patent and Implementation Acts.

Kadyrova I, Kolesnichenko S, Korshukov I, Barkhanskaya V, Sultanbekova A, Kolesnikova E, Babenko D. Certificate of registration in the state registry of rights to objects protected by copyright No. 24868 dated April 7, 2022. Development of an express method for detecting the SARS-CoV-2 virus in material obtained from a nasal swab using Time-of-Flight Mass Spectrometry and machine learning methods.

Two implementation acts of the research "Express method for detecting the SARS-CoV-2 virus in material taken from a nasal swab using Time-of-Flight Mass Spectrometry and machine learning methods" included in the list of methods of the Research Laboratory and in clinical practice of the Clinic of NСJSC «Medical University of Karaganda».

1. A.O. Omarova – PhD, Acting Head of the Department of Scientific Work of the Institute of Life Sciences NC JSC «KMU», the manager of the project. Scopus Author ID: 56099957900. Link to the profile in Scopus: https://www.scopus.com/authid/detail.uri?authorId=56099957900 Web of Science Researcher ID: AAR-6826-2020. Link to the profile in Web of Science: https://www.webofscience.com/wos/author/record/%20AAR-6826-2020ORCID ID: 0000-0002-2087-3824. Ссылка на ORCID: https://orcid.org/0000-0002-2087-3824
2. I.A. Belyaev – PhD, Associate Professor of the Department of Biomedicine NC JSC «KMU». Scopus Author ID: 55935731100. Link to the profile in Scopus: https://https://www.scopus.com/authid/detail.uri?authorId=55935731100ORCID ID: 0000-0003-3373-2059. Link to the ORCID: https://https://https://orcid.org/0000-0003-3373-2059
3. N.Zh. Yerdessov – master of Medical Sciences in “Medical and Preventative Care”, Assistant Professor at the School of Public Health NC JSC «KMU». Scopus Author ID: 57547933600. Link to the profile in Scopus:https://www.scopus.com/authid/detail.uri?authorId=57547933600 Web of Science Researcher ID: IQW-9082-2023. Link to the profile in Web of Science:https://www.webofscience.com/wos/author/record/IQW-9082-2023ORCID ID: 0000-0001-8095-3140 .Ссылка на ORCID:https://https://orcid.org/0000-0001-8095-3140
4. Ch.U. Ismailov – master of Medical Sciences in “Public Health”, Research Manager of the Sanitary and Hygienic Research Laboratory of the Institute of Public Health and Occupational Health NC JSC «KMU». ORCID ID: 0000-0002-4766-7115.Link to the ORCID:https://orcid.org/0000-0002-4766-7115
5. Zh.T. Amirhanova – master of Natural Sciences in “Biology”, Lecturer of the Department of Biomedicine NC JSC «KMU». ORCID ID: 0000-0002-5487-3574.Link to the ORCID: https://orcid.org/0000-0002-5487-3574
6. A.D. Abdrahmanov – master “Public Health Management”, Assistant of the Department of Family Medicine NC JSC «KMU».
7. A.B. Koshmaganbetova – a specialist in the block of the registrar's office of the Department of Academic Works of NC JSC «KMU»

List of publications by members of the research group

1. Omarova A., Tussupova K., Berndtsson R., Kalishev M., Sharapatova K. Protozoan Parasites in Drinking Water: A System Approach for Improved Water, Sanitation and Hygiene in Developing Countries // International Journal of Environmental Research and Public Health. –2018. – №15(3). – E495. doi:10.3390/ijerph15030495. (IF=2.44 в Web of Science and Scopus; квартиль-Q1 (процентиль-83) по общественному здравоохранению, охране окружающейсреды и гигиене труда).
2. Omarova A., Tussupova K., Hjorth P., Kalishev M., Dosmagambetova R. Water Supply Challenges in Rural Areas: A Case Study from Central Kazakhstan // International Journal of Environmental Research and Public Health. – 2019. – №16(5). – E688. doi:10.3390/ijerph16050688. (IF=2.81 в Web of Science and Scopus; квартиль-Q1 (процентиль84) по общественному здравоохранению, охране окружающей среды и гигиене труда).
3. Omarova A., Tussupova K. The future of piped water in villages in low- and middleincome countries // European Journal of Public Health. – 2018. – Vol.28, Suppl.4. – P. 336-337.doi.org/10.1093/EURPUB/CKY214.081 (IF=2.234 в Web of Science; квартиль-Q2 по общественному здравоохранению, охране окружающей среды и гигиене труда).
4. Беляев И.А., Беляев А.М Чувствительность пневмококков к антимикробным препаратам по данным исследования, проведенного в Центральном Казахстане // Georgian medical news. – 2017. – №1(262). - Р.101-106.
5. Ибраев С.А., Жарылкасын Ж.Ж., Отаров Е.Ж., Исмаилов Ч.У. Современные аспекты профессионального риска у лиц умственного труда // Международный журнал прикладных и фундаментальных исследований. – 2017. – № 3 (часть 1) – С. 62-65.
6. Anastassiya A. Mashentseva, Milana A. Ibragimova, Saule B. Akhmetova, L.Kozlovskiy, Maxim V. Zdorovets, Zhanerke T. Amirkhanova. Synthesis, radical scavenging, andantimicrobial activities of core–shell Au/Ni microtubes // Chemical Papers. – 2020. – V.74. – P.2189-2199

Based on the results obtained over the entire period of the study and their analysis, scientifically based recommendations have been compiled to improve access to appropriate WASH conditions for the prevention and control of COVID–19 in rural medical institutions.

A methodological recommendation has been developed and approved on the topic "Guidelines for improving access to adequate water supply, sanitation and hygiene (WASH), for the prevention and control of infectious diseases in rural medical institutions". This guide may be useful to residents of rural localities, which will increase their awareness in the field of WASH, through the transfer of knowledge and the development of hand-washing skills according to the protocol, in accordance with the instructions of health experts.

The target consumers are specialists of sanitary and epidemiological surveillance organizations, regional health management and environmental monitoring. In addition, the materials of the research work are also available to students and the teaching staff of «KMU» NP JSC and are used in the educational process.

Published in 2021:
1. Omarova A.O., Belyayev I.A., Akhmetova S.B., Yerdesov N.Zh., Ismailov Ch.U.,Kharin A.D. Challenges of microbiological safety of water supply, sanitation and hygiene. Literature review // Наука и Здравоохранение. - 2021. - №4 (Т.23). - С.46-57 (рекомендованный КОКСНВО МНВО РК)
2. Omarova A. Analysis of the current situation with water, sanitation and hygiene (WASH) in rural hospitals // European Journal of Public Health. - 2021. Vol.31, Suppl.3. - P. 129. doi.org/10.1093/eurpub/ckab164.340 (IF=2,391 в Web of Science; квартиль-Q1 по общественному здравоохранению, охране окружающей среды и гигиене труда)
Published in 2022:
3. Akhmetova S.B., Belyayev I.A., Omarova A.O., Amirkhanova Zh.T. The Study of Microbial Contamination of Water and Sanitary Facilities in the Karaganda Regional Hospitals; During Different Seasons // Вестник Карагандинского университета. Серия Биология. Медицина. География. – 2022. - №4(108). – С.21-30. (рекомендованный КОКСНВО МНВОРК)
4. Omarova A., Yerdessov N. Assessment of the Accessibility and Condition of Current Water, Sanitation, and Hygiene Services in Rural Medical Institutions: A Case Study from Central Kazakhstan // Proceedings of Academics World International Conference. – Paris, France, 12th –13th December, 2022. – P. 8-11.
5. Омарова А.О., Ердесов Н.Ж. Проблема обеспеченности сельского населения Республики Казахстан питьевой водой // Cборник научных трудов 9-ой республиканской научно-практической конференции с международным участием «Актуальные проблемы охраны окружающей среды и здоровье человека». - г. Ташкент, Республика Узбекистан, 2022. – С. 67-70.
Published in 2023:
6. Ердесов Н.Ж., Омарова А.О., Исмаилов Ч.У. Обеспеченность услугами водоснабжения и водоотведения в Карагандинской области // Вестник Карагандинского университета. Серия Биология. Медицина. География. – 2023. - №3(111). – С. 216-223. (рекомендованный КОКСНВО МНВО РК)

Information on the composition of the research group is presented in Table.

Table - The composition of the research group for conducting scientific research, including foreign scientists, young scientists (postdoctoral students, doctoral students, master’s, and undergraduate students)

No	Full name, education, degree, academic title	Hirsch index, identifiers
1	Ibrayeva Lyazat K., general practitioner and pulmonologist of the highest category, Doctor of Medical Sciences (M.D.), Karaganda Medical University, Department of Internal Medicine, Professor	h-index (Scopus): 2; ORCID: 0000-0002-9917-0258; Scopus Author ID: 56712888900; Researcher ID: ABZ-8951-2022
2	Molotov-Luchanskiy Vilen B., doctor-therapist of the highest category, Doctor of Medical Sciences (M.D.), Karaganda Medical University, Department of Internal Medicine, Professor	h-index (Scopus): 3; ORCID: 0000-0001-8473-4375; Scopus Author ID: 56612038200
3	Alina Asel R., rheumatologist of the highest category, Doctor of Medical Sciences (M.D.), Karaganda Medical University, Department of Internal Medicine, Professor	h-index (Scopus): 1; ORCID: 0000-0002-3229-1506; Researcher ID: ABZ-7000-2022; Scopus Author ID: 36967305200
4	Bacheva Irina V., doctor-therapist and nephrologist of the highest category, PhD, Karaganda Medical University, Department of Internal Medicine, Associate Professor	h-index (Scopus): 1; ORCID: 0000-0002-5576-8637; Researcher ID: AAR-4890-2020; Scopus Author ID: 56612421200
5	Rybalkina Dina Kh., radiology doctor, Candidate of Medical Sciences (PhD), Karaganda Medical University, Department of Internal Medicine, Associate Professor	h-index (Scopus): 1; ORCID: 0000-0002-2041-1259; Researcher ID: AFF-7942-2022; Scopus Author ID: 54890642500
6	Nygimetova Aynash A., Head of the Pulmonology Department, Regional Clinical Hospital of the Karaganda Region	ORCID: 0009-0004-5985-6257
7	Bazarova Nazira K., Acting Head of the Therapeutic Center, Regional Clinical Hospital, Cardiologist of the Highest Category, Master of Medical Sciences	ORCID: 0000-0003-4777-4620
8	Kaneyev Ilya E., radiology doctor, Karaganda Medical University, Department of Radiation Diagnostics, Assistant	ORCID: 0000-0003-3302-1062
9	Zhanabayeva Ayzhan S., pulmonologist, Karaganda Medical University	ORCID: 0000-0003-4221-9492
10	Mukhatova Aizhan Ardakovna, pulmonology resident (2nd year of training), Karaganda Medical University	ORCID: 0009-0004-2204-6808
11	Klassen Olga Sergeevna, doctoral student (2nd year of training), pulmonologist, Karaganda Medical University	ORCID: 0000-0002-9267-2968
12	Aubakirova Meruert Kairollaevna, doctoral student (2nd year of training), pulmonologist, Karaganda Medical University	ORCID: 0000-0003-4053-0260
13	Borchashvili Seda Abdul-Kadyrovna, nephrologist, University Clinic of Karaganda Medical University	ORCID: 0000-0002-8032-4819
14	Murzabayev Askhat Aidarovich, rheumatology resident (2nd year of training), Karaganda Medical University	ORCID: 0009-0003-8904-3612
15	Yuldasheva Malika Irstayevna, rheumatology resident (2nd year of training), Karaganda Medical University	ORCID: 0009-0008-5936-8500
16	Avdienko Olga Vyacheslavovna, junior researcher at the research laboratory, Karaganda Medical University	ORCID: 0000-0001-9122-1279

Scientific publications of the academic supervisor (Ibrayeva L.K.):

1. Turgunova L.G., Shalygina A.A., Ibrayeva L.K., Turmuhambetova A.A. Metabolic syndrome as a factor affecting intima-media thickness in patients with rheumatoid arthritis (Scopus citation index 1) // Open Access Macedonian Journal of Medical Sciences (General Medicine - 49th percentile), 2021, 9, pp. 411–416. DOI: doi.org.
2. Laryushina Y., Parakhina V., Turmukhambetova A., Turgunova L., Ibraeva L., Amirkhanova D., Nildibayeva F. The relationship between the level of FABP4, risks of type 2 diabetes mellitus, and cardiovascular events (PubMed citation index 4) // Open Access Macedonian Journal of Medical Sciences (General Medicine - 49th percentile), 2020, 8(B), pp. 762–768. DOI: doi.org.
3. Tulegenova D.E., Ibrayeva L.K., Rybalkina D.K., Minbayeva L.S., Bacheva I.V. Justification of the need to normalize vitamin D status for immunoprophylaxis (RSCI citation index 1) // Problems of Nutrition (General Medicine - 50th percentile), 2020, 89(6), pp. 70–81. DOI: doi.org.
4. Namazbaeva Z., Battakova S., Ibrayeva L., Sabirov Z. Change in metabolic and cognitive state among people of the Aral zone of ecological disaster (Scopus citation index 1) // Israel Journal of Ecology and Evolution (Biological Sciences – 40th percentile), 2018, 64(1-4), pp. 44–55. DOI: doi.org.
5. Grebeneva O.V., Rybalkina D.H., Ibrayeva L.K., Shadetova A.Zh., Drobchenko E.A., Aleshina N.Yu. Evaluating occupational morbidity among energy enterprise employees in industrial regions of Kazakhstan // Russian Open Medical Journal (General Medicine - 35th percentile), 2021, 10(3), e0319. DOI: doi.org.

Publications of the main staff of the research group

1. Omertayeva D., Muravlyova L., Ponomaryova O., Molotov-Luchanskiy V., Bakirova R., Klyuev D., Mugazov M. The level assessment of extracellular nucleic acids in the blood of pregnant women with chronic hypertension with superimposed preeclampsia (Scopus citation index 3) // Open Access Macedonian Journal of Medical Sciences, 2020, 8(B), pp. 514–518. DOI: doi.org.
2. Molotov-Luchanskiy V., Nukhuly A., Muravlyova L., Bakirova R., Kossybayeva A., Klyuyev D., Demidchik L., Beinikova I. Neutrophil Oxidized-Modified Proteins and Neutrophil Extracellular Traps in Patients with Community-Acquired Pneumonia // Pulmonary Medicine, 2020, 2020, 4897038. DOI: doi.org.
3. Sheryazdanova D., Laryushina Y., Vassilyeva N., Serikbaeva A., Alina A., Butyugina M., Tauesheva Z. Association between 1,5-anhydro-D-sorbitol, Insulin, and Incretins in Patients with Pre-diabetes and ST-elevation Myocardial Infarction // Open Access Macedonian Journal of Medical Sciences, 2022, 10, pp. 464–469. DOI: doi.org.
4. Sheryazdanova D.N., Laryushina Y.M., Muravlyova L.Y., Turgunova L.G., Alina A.R., Vassilyeva N.V., Turmukhambetova A.A. Insulin resistance and contrainsular response in type 2 diabetes mellitus patients with acute coronary syndrome // Open Access Macedonian Journal of Medical Sciences, 2020, 8(B), pp. 943–948. DOI: doi.org.
5. Rybalkina D.K., Ibrayeva L.K., Salimbaeva B.M., Minbayeva L.S., Bacheva I.V., Drobchenko Y.A., Aleshina N.Y. Assessment of medical losses due to socially significant diseases in the East Kazakhstan Region // Przeglad Epidemiologiczny, 2020, 74(3), pp. 543–549. DOI: doi.org.
6. Ibrayeva L.K., Rybalkina D.Kh., Zhanbasinova N.M., Drobchenko E.A. Medical and social losses from diseases of the circulatory system in industrial regions of Kazakhstan // Russian Journal of Occupational Health and Industrial Ecology, 2019, 59(7), pp. 444–448. DOI: doi.org.
7. Bacheva I., Umbetalina N., Bregvadze-Tabagari N., Shalygina A., Baidildina B. Epidemiology, structure, and algorithm of management of pregnant women with extragenital pathology of therapeutic profile (Scopus citation index 1) // Georgian Medical News, 2017, (264), pp. 25–31. PMID: 28480844.
8. Bacheva I.V., Umbetalina N.S., Akhmaldinova L.L. The possibilities of serum cystatin C in the diagnosis of pyelonephritis in pregnant women (Scopus citation index 1) // Klinichescheskaya Laboratornaya Diagnostika, 2016, (11), pp. 772–776. DOI: doi.org.
9. Koichubekov B., Sorokina M., Korshukov I., Bakirova R., Bekov E., Omarbekova N. Psychophysiological status and life quality in individuals with metabolic syndrome living in central Kazakhstan // Open Access Macedonian Journal of Medical Sciences, 2020, 8(B), pp. 463–469. DOI: doi.org.
10. Klassen O.S., Shatko V.E., Kulymbetov A.K., Bacheva I.V., Ibrayeva L.K., Rybalkina D.Kh. Chronic kidney disease and acute kidney injury outcomes in COVID-19 // Materials of the XXVII All-Russian Scientific and Practical Conference with International Participation. Clinical Laboratory: Contribution to Combating the Pandemic. Collection of Abstracts. Moscow, 2022, pp. 62–63.
11. Aliyakparov M.T., Turmukhambetova A.A., Kaneev I.E. Radiological diagnostic methods in COVID-19 // Medicine and Ecology, 2021, No. 4 (101), pp. 5–7.
12. Baidildina B.N., Turgunova L.G., Bacheva I.V., Akhmaldinova L.L., Laryushina E.M. Association of "traditional" risk factors and CXCL16 chemokine levels in the development of cardiovascular events // Medicine and Ecology, 2019, No. 1 (90), pp. 92–98.
13. Zhanabaeva A.S., Sagadi N.M. (Pulmonology Residents), Ibrayeva L.K. (Supervisor), et al. Increased aging rate as a factor exacerbating the course of COVID-19 // Materials of the II National Congress with International Participation on Human Ecology, Hygiene, and Environmental Medicine "Sysinsky Readings - 2021", November 17-19, 2021, Moscow, pp. 160–165. ISBN 978-5-9500159-4-6. (RSCI). doi.org.

A total of 150 patients with chronic lung diseases (CLD) (75 patients with chronic obstructive pulmonary disease (COPD) and 75 patients with interstitial lung disease associated with systemic sclerosis (ILD-SS)) participated in a three-year longitudinal study. To study the clinical and functional parameters in individuals with CLD with and without chronic heart failure (CHF) and impaired renal function (IRF), all patients were divided into groups: group 1 (without CHF and without IRF), group 2 (with CHF without IRF), group 3 (with IRF without CHF) and group 4 (with CHF and with IRF). The assessment was carried out using a comprehensive approach, including clinical and functional tests (6-minute walk test (6MWT), spirometry, capnography, echocardioscopy (ECHOKS), heart rate variability (HRV)), instrumental methods (chest computed tomography (CT) and densitometry), biochemical markers (endothelin-1, galectin-3, NT-proBNP, MR-proANP, hsTnT, blood and urine creatinine, urine albumin, GFR, albumin-creatinine ratio, blood acid-base balance), and quality of life assessment were also assessed. Statistical analysis included correction for multiple comparisons (FDR) to minimize false-positive results.

Analysis of the dynamics of four clinical groups showed the conditional development of two stages of the disease course. In the period 2023-2024, destabilization predominated: preservation of the initial phenotype was observed in only 60% of patients with COPD and in 54% with ILD-SS. Transitions from group 1 to more severe ones (development of CHF and/or IRF) were predominant. in 48% of patients with COPD and 56% of patients with ILD-SS), with a decrease in the proportion of patients without complications (in COPD from 36% to 9.5%, in ILD-SS from 33.3% to 3.4%). The key pattern of this period was the transition from group 2 to group 4 (27% in COPD and 32.5% in ILD-SS), which can likely be regarded as a cumulative increase in polymorbid conditions in the lung-heart-kidney relationship. In 2024–2025, stabilization was noted (87% of patients with COPD and 91.5% of patients with ILD-SS remained in the original groups), however, the number of patients in group 4 increased in the cohort structure (COPD to 49%; ILD-SS to 48.4%).

There were no intragroup differences in quality of life (q> 0.68 for COPD; q>0.8 for ILD-SS), with consistently low indicators in all groups. The most pronounced impairments were recorded in the area of social support due to dyspnea and functional limitations. Internosologically, in group 2 (with CHF), patients with ILD-SS demonstrated better indicators of overall quality of life and self-perception (q<0.05), possibly due to differences in the perception of restrictive pathology. In group 4, the differences leveled out, with the exception of a trend towards better social well-being in ILD-SS (insignificant after FDR). The general trend is higher quality of life in ILD-SS, especially with cardiac comorbidity.

Analysis of the dynamics of pulmonary profile indicators in patients with ILD-SS and COPD showed that both nosologies demonstrate an annual deterioration in pulmonary function and exercise tolerance, but along different trajectories of the continuum: in COPD, significantly worse functional parameters: lower oxygen saturation (SаO₂) before/after 6MWT, higher respiratory rate and dyspnea severity according to Borg, higher heart rate, lower FEV₁ and FVC (all differences are clinically significant, most are statistically significant after FDR); in ILD-SS, there is predominantly structural progression according to CT densitometry data with a decrease in lung volume and an increase in the density of the lung tissue parenchyma (less negative HU), especially in combined CHF + IFR (group 4), where this effect is preserved taking into account multiple comparisons. It should be noted that patients with ILD-SS initially had smaller lung volumes according to CT densitometry data compared to patients with COPD.

Comparisons within nosological groups by subgroups with CHF and IFR are generally minimal and unstable after FDR; the functional status profile is similar, with typical signs of limited exercise tolerance (desaturation, increased RR/HR, increased dyspnea after 6MWT). In ILD-SS, there is a tendency toward worse indicators in group 4 (CHF+IFR), where higher blood lactate values, lower FEV₁/FVC, and a more frequent increase in RR were detected.

Analysis of the dynamics of the cardiac profile showed that in COPD, HRV is stable regardless of comorbidity, with trends toward increased MR-proANP and NT-proBNP in groups 2 and 4 (q > 0.05 after FDR), indicating subclinical atrial strain. In ILD-SS, trends toward increased HF and decreased LF were observed in group 4 (loss of significance after FDR). Internosologically, in group 2, HF is higher in COPD (q=0.00), VLF is higher in ILD-SS (q=0.02); in group 4, the differences level out. Longitudinal dynamics: decrease in TP and HF by 2025, increase in NT-proBNP, hsTnT and MR-proANP (higher in ILD-SS), moderate decrease in left ventricular ejection fraction (LV EF) and increase in pulmonary artery systolic pressure (PASP, significantly higher in COPD, q≈0.033). Natriuretic peptides are sensitive markers of subclinical dysfunction; restrictive pathology increases atrial load, obstructive pathology increases pulmonary hypertension.

An analysis of the nephrological profile revealed that in Group 4, patients with CHF and NF-C had consistent differences in the increase in blood creatinine, urine albumin-to-creatinine ratio, and decrease in GFR, even after adjusting for multiple comparisons in both nosologies. Internosological comparisons revealed the following trend: in Groups 2 and 4, patients with COPD had statistically significantly higher blood creatinine values and a decrease in GFR, without any changes in kidney structure according to CT densitometry. Moreover, the annual GFR decline was approximately 6%. In patients with ILD-SS, the baseline SCF level was lower, but the annual GFR decline was more gradual, at 3%. A similar trend was also observed for the albumin-to-creatinine ratio.

Analysis of the dynamics of changes in galectin-3 and endothelin-1 levels in patients with ILD-SS revealed an increasing trend in groups 3 and 4, which was lost thereafter. A comparative analysis of these markers between the two nosologies showed that galectin-3 was higher in patients with ILD-SS, especially in group 4 (p=0.04, q=0.10), while endothelin-1 was higher in patients with COPD. Correlation analysis revealed positive associations between endothelin-1 and the cardiac subprofile C3 in patients with COPD, and between galectin-3 and the pulmonary subprofiles R1, R7, and R9–R11 in patients with ILD-SS. Negative correlations of endothelin-1 with SaO₂ and lung volumes emphasize vasoconstriction in hypoxemia. The dynamics of changes in the profiles showed that the pulmonary profile had minimal internosological differences (|Cliff’s δ| ≤ 0.07; q ≥ 0.64), with a tendency to worsen in COPD; the cardiology profile had initially low indicators in ILD-SS (δ ≈ 0.26–0.27; q ≈ 0.04–0.05), which somewhat leveled out in the dynamics by 2025; the nephrology profile was initially worse in ILD-SS (δ ≈ 0.68–0.69; q ≈ 0), but by 2025 there was a more negative dynamics in patients with COPD. The dynamics by subprofiles demonstrated the same trajectories: in COPD — inflammatory-endothelial deviations, especially in nephrology indicators by 2025; In ILD-SS, a fibro-dominant pattern is observed. Correlations between profiles are low, indicating domain autonomy.

Thus, the obtained results support the hypothesis of vascular imbalance as a key factor: endothelin-1 is an indicator of vascular-cardiac stress (predominant in COPD, exacerbating hypertension and hypoxemia); galectin-3 is a marker of fibrosis and functional limitations (relevant in ILD-SS, exacerbating restrictive changes). Comorbidity (CHF/IFR) amplifies the imbalance, promoting the progression of the pulmonary-cardiorenal continuum through inflammation, hypoxia, and remodeling.

Field of Application: Medical organizations and medical education institutions.

The impact of the obtained results on the development of the main research area and related fields of science and technology: achieving the project's goal will help develop an understanding of the key mechanisms underlying the pathogenesis of vascular dysfunction within the pulmonary-cardiorenal continuum, which could improve the early diagnosis and treatment of organ damage. Improving the pathogenetic mechanisms underlying the development of organ dysfunction within the pulmonary-cardiorenal continuum could enable an effective search for solutions to prevent adverse outcomes and reduce the socioeconomic burden of disease.

Potential for the use of the results: The developed pulmonary-cardiorenal continuum model enables physicians to conduct risk stratification and personalized management of patients with COPD and ILD-SS. The data have been implemented in clinical practice at three medical organizations. Three intellectual property rights (models and algorithms) have been obtained, confirming the high practical potential. Publications in Scopus and KOKSNVO journals ensure scientific representativeness, and the results can be used in the development of clinical guidelines and educational programs.

Implementation status: Research results have been implemented in practical healthcare at the Regional Clinical Hospital of the Healthcare Institution of the Karaganda Region (3 implementation certificates dated October 3, 2025), Karaganda Polyclinic No. 15 (3 implementation certificates dated October 2, 2025), and in the educational process at the Department of Internal Medicine of the Karaganda State Autonomous Okrug (3 implementation certificates dated October 3, 2025).

1. Ibraeva L.K., Alina A.R., Bacheva I.V., Rybalkina D.Kh., Borchashvili S.A.K., Murzabayev A.A., Zhanabaeva A.S., Klassen O.S., Aubakirova M.K., Mukatova A.A. Heart rate variability in patients with interstitial lung diseases // Pharmacy of Kazakhstan. - 2024. - No. 4 (255). - pp. 84–90. DOI: 10.53511/pharmkaz.2024.78.19.010. (KOKSON)
2. Afizov I.E., Aubakirova M.K.; scientific supervisors: Bazarova N.K., Ibraeva L.K., Rybalkina D.Kh. Analysis of the relationship between echocardiographic parameters and markers of cardiac dysfunction in patients with chronic lung diseases // Proceedings of the LXIII International Scientific Conference of Young Scientists «Science: Yesterday, Today, Tomorrow», West Kazakhstan Medical University named after M. Ospanov, Aktobe, April 11-12, 2024, pp. 277–278. (Abstract). The authors were awarded 2nd-degree diploma for their presentation in the "Young Scientists in Clinical Medicine" section.
3. Mukatova A.A. Analysis of quality of life in patients with chronic lung diseases // Proceedings of the International Scientific Conference «University Science: A Look into the Future», dedicated to the 89th anniversary of Kursk State Medical University, Kursk, February 8-9, 2024, pp. 555–557. (Abstract).
4. Ibrayeva L.K., Rybalkina D.Kh., Alina A.R., Bacheva I.V., Aubakirova M.K., Klassen O.S., Ovtsynov P.V. Bioimpedancemetry in patients with chronic lung diseases in the context of the pulmonary cardiorenal continuum // Voprosy pitaniia. Vol. 93, No. 6, 2024, pp. 105-116. www.scopus.com (CiteScore 2023 Medicine 47 percentile)
5. L.K. Ibrayeva , O.S. Klassen, A.R. Alina, I.V. Bacheva, A.A. Nygimetova. РULMONARY-RENAL INTERACTIONS IN CHRONIC LUNG DISEASES — CURRENT STATE OF THE PROBLEM OBLEM // Phthisiopulmonology. №4 (46) 2024, С.152-159. DOI: 10.26212/2227-1937.2025.84.83.020
6. L.K. Ibraeva, M.K. ASSOCIATION OF FUNCTIONAL STATUS OF THE MYOCARDIAL AND REMODELING OF RESPIRATORY ORGANS IN CHRONIC LUNG DISEASES (review article) // Phthisiopulmonology. No. 4 (46) 2024, pp. 48-54. DOI: 10.26212/2227-1937.2025.41.22.007L.K. Ibraeva, M.K. ASSOCIATION OF FUNCTIONAL STATUS OF THE MYOCARDIAL AND REMODELING OF RESPIRATORY ORGANS IN CHRONIC LUNG DISEASES (review article) // Phthisiopulmonology. No. 4 (46) 2024, pp. 48-54. DOI: 10.26212/2227-1937.2025.41.22.007
7. Ibrayeva L, Aubakirova M, Bacheva I, Alina A, Bazarova N, Zhanabayeva A, Avdiyenko O, Borchashvili S, Tazhikhanova S, Murzabaeyev A. Features of Heart Failure with Preserved Ejection Fraction in Patients with Chronic Obstructive Pulmonary Disease and Systemic Sclerosis-Associated Interstitial Lung Diseases.// Journal of Personalized Medicine. 2025; 15(5):206. doi.org (CiteScore 2024 Medicine (miscellaneous) (79th percentile).
8. Ibrayeva, L.; Bacheva, I.; Alina, A.; Klassen, O. Assessing Fibrosis Progression and Endothelial Dysfunction in SSc-ILD and COPD: An Integrated Biomarker and CT Densitometry Approach.// Medicina 2025, 61(9), 1572. doi.org (Рейтинг CiteScore 2024 General Medicine 81 процентиль). (CiteScore 2024 General Medicine (81st percentile).
9. Ibrayeva, L.; Bacheva, I.; Alina, A.; Klassen, O. Pulmo–Cardio–Renal Continuum in Chronic Lung Diseases: A 3-Year Prospective Cohort Study.// Journal of Clinical Medicine. 2025, 14, 7631. doi.org (CiteScore 2024 General Medicine 86th percentile).

Three intellectual property certificates were received:

1. No. 62075 dated September 12, 2025. Phenotypic features of the development of chronic heart failure with preserved ejection fraction in patients with chronic obstructive pulmonary disease and interstitial lung diseases. Authors: Aubakirova M.K., Ibrayeva L.K., Bacheva I.V., Alina A.R., Rybalkina D.Kh.
2. No. 62077 dated September 12, 2025. An integrated approach to assessing the pulmonary-renal continuum in chronic lung diseases. Authors: Klassen O.S., Ibrayeva L.K., Bacheva I.V., Alina A.R., Nygimetova A.A., Kaneev I.E.
3. No. 62108 of September 15, 2025. Model of the pulmonary-cardiorenal continuum in chronic respiratory diseases. Authors: Ibrayeva L.K., Bacheva I.V., Klassen O.S., Alina A.R.

Results Presented at Conferences and Congresses:

1. Lyazat Ibrayeva. Analysis of antifibrotic therapy use in patients with progressive pulmonary fibrosis in Karaganda Region: oral presentation // X Congress "Innovative Technologies in Respiratory Medicine." – Astana, October 19-21, 2023.
2. Lyazat Ibrayeva. Progressive pulmonary fibrosis: new paradigms from the pulmonologist's perspective: oral presentation // Scientific-Practical Conference dedicated to the 65th anniversary of the Institute of Public Health and Occupational Medicine with international participation, "Prospects for the Development of Occupational and Environmental Medicine." – Karaganda, November 2–3, 2023.
3. Lyazat Ibrayeva, Irina Bacheva, Assel Alina, Olga Klassen, Meruyert Aubakirova. Computed tomography of the lungs in chronic lung diseases: poster presentation // The Book of Abstracts from the 22nd European Congress of Internal Medicine. – Istanbul, March 6–9, 2024 (presented by Meruyert Aubakirova).
4. Lyazat Ibrayeva, Irina Bacheva, Assel Alina, Olga Klassen, Meruyert Aubakirova. Serpine1 (PAI-1) gene polymorphism in chronic lung diseases: poster presentation // The Book of Abstracts from the 22nd European Congress of Internal Medicine. – Istanbul, March 6–9, 2024 (presented by Olga Klassen).
5. A.V. Bekenova, Z.Zh. Ualikhanova, S.S. Tazhikhanova, A.A. Mukatova. Interstitial lung disease in systemic scleroderma: oral presentation // International Student Conference «Fundamental and Clinical Aspects of Rare Diseases». – Almaty, February 29, 2024 (presented by A.V. Bekenova and A.A. Mukatova).
6. Lyazat Ibrayeva. Aspects of diagnosis and antifibrotic therapy for idiopathic pulmonary fibrosis: oral presentation // VII International Scientific and Practical Conference «Current Issues in Diagnosis and Treatment of Respiratory Pathology». – Almaty, October 16, 2024.
7. Lyazat Ibrayeva. Monitoring patients with interstitial lung diseases at various stages of medical care: oral presentation // XI Congress «Innovative Technologies in Respiratory Medicine». – Astana, October 24–26, 2024.
8. Meruyert Aubakirova, Saltanat Tazhikhanova. Diagnostic significance of cardiac markers in patients with interstitial lung diseases: poster presentation, supervised by Lyazat Ibrayeva // XI Congress «Innovative Technologies in Respiratory Medicine». – Astana, October 24–26, 2024.
9. Olga Klassen, Aizhan Mukatova. HIV, tuberculosis, and pneumocystosis: oral presentation, supervised by Lyazat Ibrayeva // XI Congress «Innovative Technologies in Respiratory Medicine». – Astana, October 24–26, 2024.
10. Meruyert Aubakirova. COPD and CHF: from biomarkers to predictions: oral presentation // III National Congress on Respiratory Medicine with international participation «Respiratory Medicine in Kazakhstan: A Course for Integration and Cooperation». – Astana, December 5–7, 2024. (1rd place in the competition for young scientists).
11. Olga Klassen. Assessment of sarcopenia in patients with chronic lung diseases: oral presentation // III National Congress on Respiratory Medicine with international participation «Respiratory Medicine in Kazakhstan: A Course for Integration and Cooperation». – Astana, December 5–7, 2024. (3rd place in the competition for young scientists).
12. Lyazat Ibrayeva. Analysis of antifibrotic therapy use in patients with progressive pulmonary fibrosis in Karaganda Region: oral presentation // III National Congress on Respiratory Medicine with international participation «Respiratory Medicine in Kazakhstan: A Course for Integration and Cooperation». – Astana, December 5–7, 2024.
13. Lyazat Ibrayeva. Analysis of antifibrotic therapy use in patients with progressive pulmonary fibrosis in Karaganda Region: oral presentation // XII CONGRESS INNOVATIVE TECHNOLOGIES IN RESPIRATORY MEDICINE. III REPUBLICAN FORUM "CYSTIC FIBROSIS: PRESENT AND FUTURE". – Astana, April 17-19, 2025.
14. Olga Klassen. Body Composition Assessment in Patients with Chronic Lung Diseases Based on Bioelectrical Impedance Analysis (InBody) // VII Central Asian International Conference on Medical Education MEDICAL EDUCATION 4.0: GLOBAL TRENDS AND LOCAL SOLUTIONS, dedicated to the 75th anniversary of the Karaganda Medical University and the International Year of Glaciers’ Preservation 2 – 3 June 2025.
15. Meruyert Aubakirova. Clinical use of Varicard in patients with chronic lung diseases in combination with early manifestations of cardiopathology // VII Central Asian International Conference on Medical Education MEDICAL EDUCATION 4.0: GLOBAL TRENDS AND LOCAL SOLUTIONS, dedicated to the 75th anniversary of the Karaganda Medical University and the International Year of Glaciers’ Preservation 2 – 3 June 2025.
16. Oral presentation (offline) by O.S. Klassen, "An Integral Approach to Chronic Lung Diseases: CT Densitometry and Biomarkers in the Context of Renal Dysfunction," at the XXXV National Congress on Respiratory Diseases, Moscow, October 14-17, 2025.
17. Oral presentation (offline) by L.K. Ibrayeva, "Diagnostics and Treatment of ILD: Karaganda Region," at the IV National Congress of Respiratory Medicine with International Participation, "Respiratory Medicine of Kazakhstan: Breath of the Future," Astana, November 13-15, 2025.
18. Oral presentation (offline) by I.V. Bacheva "Phenotypic Features of the Development of the Pulmonary-Cardiorenal Continuum in Patients with COPD" at the IV National Congress of Respiratory Medicine with International Participation "Respiratory Medicine of Kazakhstan: Breath of the Future", Astana, November 13-15, 2025.
19. Oral presentation (offline) by Zhanabaeva A.S. "Comprehensive Assessment of Dynamic Changes in the Structural and Functional Profile of the Lungs, Markers of Fibrosis and Endothelial Dysfunction in Chronic Lung Diseases Taking into Account the PAI-1 Genotype" at the IV National Congress of Respiratory Medicine with International Participation "Respiratory Medicine of Kazakhstan: Breath of the Future", Astana, November 13-15, 2025 (2nd prize in the young scientists competition).
20. Oral presentation (offline) by Aubakirova M.K. "Three-Year Dynamics of Interstitial Pulmonary Fibrosis and Cardiovascular Changes in Patients with ILD-SS" at the IV National Congress of Respiratory Medicine with International Participation "Respiratory Medicine of Kazakhstan: Breath of the Future", Astana, November 13-15, 2025 (1st prize in the young scientists' competition).
21. Oral presentation (offline) Bacheva I.V., Ibrayeva L.K. "Pulmo-cardio-renal continuum: new aspects of interorgan interactions" at the Central Asian Congress of Nephrologists "Modern Opportunities and Priority Areas in Nephrology", Almaty, December 4-5, 2025.
22. Oral presentation (offline) Klassen O.S. "Three-year dynamics of renal function in patients with COPD and SSc-ILD" at the Central Asian Congress of Nephrologists "Modern Opportunities and Priority Directions in Nephrology", Almaty, December 4-5, 2025 (awarded a diploma in the nomination "Scientific Novelty and Originality").

1. Turgunov Y.M. - the head of the project, doctor of medical sciences, Vice-Rector for Scientific and Clinical Work of NJSC MUK; ResearcherID R-2915-2016, Scopus Author ID - 57215313148, https://orcid.org/0000-0002-6486-3847
2. Shakeyev K. T. - major researcher of the department of surgical diseases NJSC MUK; Scopus Author ID- 56801885500 https://orcid.org/0000-0002-7802-1464
3. Kadyrova I.A. – r., PhD, associate professor of RL of IoLS of NJSC MUK; Scopus Author ID– 56297966100, https://orcid.org/0000-0001-7173-3138
4. Avdienko O.V. – junior researcher of RL of IoLS of NJSC MUK; Scopus Author ID -57221804349, https://orcid.org/0000-0001-9122-1279
5. Matyushko D. N. - r., PhD, the head of the surgical service of the UC on REM "Multidisciplinary hospital No. 1 of Karaganda" HDKR; ResearcherID: AFX-9845-2022, Scopus Author ID- 57193721109,https://orcid.org/0000-0002-7884-0507
6. Mugazov M. M. - r., PhD, аss. professor of the Department of Emergency Medical Aid, Anesthesiology and Intensive Care; ResearcherID: ADC-0851-2022, Scopus Author ID –57209321668, https://orcid.org/0000-0002-7739-8999
7. Zhumakaev Aю M. - r., the head of the Department of Abdominal Surgery of UC on REM "Multidisciplinary hospital No. 1 of Karaganda" HDKR (oncological center)
8. Ogizbayeva A. V. – r., PhD, аss. professor of the Department of Emergency Medical Aid, Anesthesiology and Intensive Care, ResearcherID:ABX-6274-2022, Scopus Author ID –57331445400,https://orcid.org/0000-0003-1006-1870

Immunological studies were carried out for the detection of markers of bacterial translocation LBP, sCD14-ST in blood serum by ELISA and molecular genetic studies for the detection of bacterial translocation in mesenteric lymph nodes in studied patients, based on the results of which a preliminary statistical and complex analysis were carried out

Published 3 article in a peer-reviewed scientific publication with a CiteScore percentile in the Scopus database of at least 35 %

1 article was published in a peer-reviewed domestic publication recommended by CECSES -Journal of Clinical Medicine of Kazakhstan.

Received 3 certificates of entering information into the state register of rights to objects protected by copyright.

Presented 7 reports at international and republican conferences

Applicability of the obtained scientific results: the proposed methods for detecting biomarkers will allow further studies of bacterial translocation. Some of diagnostic methods can be used in practical health care. Improving the image of Collective Use Laboratory will help to attract investment and conduct research by the scientific groups of Kazakhstan and foreign partners. The development of modern high-tech research methods will create the prospect of commercialization in the form of creating a learning platform (master classes, advanced training, specialization in the workplace, etc.).

Target consumers of results: researchers, doctors; patients with complications of bacterial translocation.

1. Огизбаева А.В., Савазова К.С., Шакеев К.Т., Тургунов Е.М. Динамика пресепсина (sCD14) у оперированных пациентов с колоректальным раком // VІІ Конгресс хирургов Казахстана с международным участием «ХИРУРГИЯ: ВЧЕРА, СЕГОДНЯ, ЗАВТРА» посвященного 75-летию со дня основания национального научного центра хирургии им. А.Н. Сызганова / г. Алматы (30 сентября - 1 октября 2021 г.) / Вестник хирургии Казахстана. – 2021. - Спец. выпуск №1. - С. 176-177.
2. Шакеев К.Т., Кабилдина Н.А., Огизбаева А.В., Тургунов Е.М., Жумакаев А.М., Елубайулы А.Е., Муратулы А.М. Динамика липополисахарид-связывающего белка (LBP) у оперированных пациентов с колоректальным раком // Сборник тезисов VIII Съезда онкологов и радиологов Казахстана с международным участием / г. Туркестан, (14-16 октября 2021г.). / Онкология и радиология Казахстана. – Спец. выпуск. – 2021. - С. 108
3. Alina Ogizbayeva, Yermek Turgunov, Irina Kadyrova, Kayrat Shakeyev, Svetlana Kolesnichenko, Miras Mugazov, Ilshat Moldozhanov. The 16s ribosomal ribonucleic acid microorganisms’ detection in mesenteric lymph nodes by a polymerase chain reaction in view of colorectal cancer // Journal of clinical medicine of Kazakhstan. – 2022. – V.19(2). – P.38-42. DOI: https://doi.org/10.23950/jcmk/11937 Журнал входит в список ККСОН с 2021 года решение КОКСОН №190 от 02.03.2021.
4. Kayrat Shakeyev, Yermek Turgunov, Alina Ogizbayeva, Olga Avdiyenko, Miras Mugazov, Sofiko Grigolashvili, Ilya Azizov. Presepsin (sCD14-ST) as a risk factor for the development of infectious and inflammatory complications in operated colorectal cancer patients // Annals of Coloproctology. – 2022. – V.38(6). – P.442-448. DOI: https://doi.org/10.3393/ac.2022.00115.0016 ISSN:2287-9714, Scopus на 2023 год– Q2, 64%
5. Огизбаева А.В., Кадырова И.А., Тургунов Е.М., Шакеев К.Т., Колесниченко С.И., Мугазов М.М. Количественное определение микроорганизмов в мезентериальных лимфатических узлах при колоректальном раке методом полимеразной цепной реакции. Свидетельство о внесение сведений в государственный реестр прав на объекты, охраняемые авторским правом № 22807 от 10 января 2022 года.
6. Огизбаева А.В., Тургунов Е.М., Авдиенко О.В., Мугазов М.М., Асамиданова С.Г., Комаров Т.В. Методика расчета риска развития неблагоприятных исходов оперативного вмешательства у пациентов с колоректальным раком. Свидетельство о внесение сведений в государственный реестр прав на объекты, охраняемые авторским правом № 29343 от 11 октября 2022 года.
7. Alina Ogizbayeva, Yermek Turgunov, Olga Avdiyenko, Kayrat Shakeyev, Miras Mugazov. sCD14-ST (presepsin) as a risk factor for the development of post-operative infection complications in colorectal cancer patients // 32nd European Congress of Clinical Microbiology & Infectious Diseases. - Poster Session PS056 - 8c. Surgical site infection. - Presentation Number: P1686. – Abstract Number: 00482. - Lisbon, Portugal, 23 – 26 April 2022.
8. Alina Ogizbayeva, Yermek Turgunov, Miras Mugazov, Iliya Azizov. Lipopolysaccharidebinding protein as a risk factor for development of infectious complications in operated colorectal cancer patients // International Surgical Week ISW 2022. - Vienna, Austria, 15-18 August 2022. -Abstract Volume ISW 2022. - Number: PE087.
9. Огизбаева А.В., Кадырова И.А., Тургунов Е.М., Шакеев К.Т., Мугазов М.М. Количественное определение ДНК бактерий в мезентериальных лимфатических узлах при колоректальном раке методом полимеразной цепной реакции // IІІ Съезд хирургов Казахстана с международным участием «Актуальные вопросы хирургии и трансплантологии» / г. Алматы (2-3 сентября 2022 г.) / Вестник хирургии Казахстана. –2022. - Спец. выпуск №1. - С. 185-186.
10. Turgunov Ye., Ogizbayeva A., Avdiyenko O., Mugazov M., Shakeyev K., Komarov T., Asamidanova S. The sCD14-ST predictive value in the development of adverse outcomes in operated colorectal cancer patients (diagnostic study) // Annals of Medicine and Surgery. – 2023. – V.85. – P.796–801. DOI: http://dx.doi.org/10.1097/MS9.0000000000000426 E-ISSN:2049-0801, Scopus на 2023 год – Q3, 45%
11. Ye. Turgunov, A. Ogizbayeva, K. Shakeyev, M. Mugazov, L. Akhmaltdinova, Sh. Nuraly, V. Rudolf. The Dynamics of the Lipopolysaccharide-Binding Protein (LBP) Level in Assessing the Risk of Adverse Outcomes in Operated Colorectal Cancer Patients // Asian Journal of Surgery. –2023. – https://doi.org/10.1016/j.asjsur.2023.08.101 https://www.scopus.com/record/display.uri?eid=2-s2.0-85169506634&origin=resultslist&sort=plf-f ISSN:1015-9584E-ISSN:0219-3108, Scopus на 2023 год – Q2, 74%
12. Огизбаева А.В., Тургунов Е.М. Маркеры бактериальной транслокации в прогнозировании исходов хирургии колоректального рака и острой кишечной непроходимости. Монография / ТОО «Типография АРКО», Караганда, 2023 – 149с. (Утверждено и разрешено к изданию типографским способом Сенатом НАО «КМУ», протокол заседания №9 от 25.05.2023 года)
13. Огизбаева А.В., Тургунов Е.М. Прогностическая значимость sCD14-ST в развитии неблагоприятных исходов у пациентов, оперированных по поводу колоректального рака // Международная научно-практической конференция «Медицина завтрашнего дня: научное наследие академика М.А. Алиева», посвященной 90-летию академика М.А. Алиева и 30-летию Казахстанско-Российского медицинского университета/ г. Алматы (19 июня 2023 г.). – секция «Хирургия и Стоматология».
14. Alina Ogizbayeva, Yermek Turgunov, Kayrat Shakeyev, Miras Mugazov, Lyudmila Akhmaltdinova. Lipopolysaccharide-Binding Protein in Assessing the Risk of Adverse Outcomes in Operated Colorectal Cancer Patients // The 56th European Society for Surgical Research congress / Lille, France ( 29-30 June, 2023). – section «Colorectal Surgery».

1. Mindubayeva F. – scientific supervisor of the project, Doctor of Medical Sciences, Professor of the Department of Physiology of «KMU» ; NCJSC.H-index : Scopus –3
2. Tukbekova B. - Doctor of Medical Sciences, Professor, Head of the Department of Pediatrics and Neonatology of «KMU» ; NCJSC.H-index – 1ORCID ID 0000-0002-9115-8510
3. Akhmaltdinova L. - Candidate of Medical Sciences, Associate Professor, Institute of Life Sciences of «KMU» ; NCJSC.H-index: Scopus – 4 Author ID в Scopus 56285614700 ORCID ID 0000-0001-5602-6136 Researcher ID in Publons B-8152-2018
4. Bolatbekuly Zh. - Candidate of Medical Sciences, head of the children s cardiac surgery department of the Municipal State Enterprise "Multiprofile Hospital No. 2 of Karaganda".»
5. Salikhova Y. Candidate of Medical Sciences, Associate Professor of the Department of Physiology of «KMU» ; NCJSC.H-index: Scopus – 1 Scopus Author ID: 57214714647 ORCID ID 0000-0001-8692-2425
6. Niyazova Y. - ass. professor of the Department of Physiology of «KMU» ; NCJSC.H-index: Scopus – 1 Scopus Author ID: 57191076751ORCID ID 0000-0002-6609-330
7. Akhmetova M. - ass. professor of the Department Physiology of of«KMU» ; NCJSC.ORCID ID 0000-0001-8357-360
8. Ospanova M. - 3rd year PhD student of «KMU» ; NCJSC.ORCID ID 0000-0002-5247-3921
9. Avdienko O. - junior researcher of research laboratory of Institute of Life Sciences of «KMU» ; NCJSC.H-index: Scopus – 1 ORCID ID 0000-0001-9122-1279
10. Akzhanov G. – junior researcher, resident of the children s cardiac surgery department of the Municipal State Enterprise Multiprofile Hospital No. 2 of Karaganda .ORCID ID 0000-0001-9352-8725

The parameters of the serotonin system in children with congenital heart disease complicated by PAH were studied before and after surgical correction: the number of platelets in the blood and their characteristics (average platelet volume, coefficient of variation of platelets by volume, thrombocrit), concentration of serotonin in blood plasma and platelets, serotonin metabolite 5 -hydroxyindoleacetic acid (5-HIAA) in blood plasma, urine and platelets; the serotonin transporter SERT in platelets and the level of 5-HT2A receptors for serototonin in platelets. A statistical analysis of the obtained data was carried out. Serotonin concentration in platelets was three times higher in children with PAH compared to the control group. In patients with severe PAH, serotonin concentration decreased by 20% after surgical treatment, suggesting serotonin s role in PAH development and pulmonary vessel remodeling. Urine tests for 5-hydroxyindoleacetic acid (5-HIAA) showed a 20-fold increase in patients with CHD, which decreased after surgical treatment. Regression analysis revealed a significant correlation between plasma 5-HIAA levels and estimated mean pulmonary arterial pressure. The study demonstrated that serotonin transporter (SERT) was significantly increased in platelets of children with CHD and decreased after surgical correction. Correlation analysis revealed significant relationships between SERT, 5-HT2A, and 5-HIAA levels in platelets, indicating the involvement of the serotonin system in PAH development.

Based on the results obtained:Scientific supervisor Mindubayeva F.A. at the XXIV Congress of the Physiological Society named after. I.P. Pavlova (September 11-15, 2023, St. Petersburg) participated with a report on the topic “The influence of excess serotonin in the embryonic period of ontogenesis on the activity of the membrane serotonin transporter in early postnatal ontogenesis.”

Scientific supervisor of the project Mindubaeva F.A. and PhD student Ospanova M.D. took part in the 34th European Pediatrics Conference (Zurich, Switzerland, October 9-10, 2023) with the presentation “Study of serotonin metabolism in children with congenital heart defects.”

Target consumers are first of all, parents of children with CHD, who, already at the initial pilot stage of the application of this technology, will be ready to conduct an examination through private clinical diagnostic centers The proposed diagnostic method can be introduced into the standards for examining preschool children and will be in demand in clinical diagnostic centers.

1. Bilalova D., Mindubaуeva F., Nigmatullina R., Salikhova Y. Monocrotaline model of pulmonary hypertension in immature rats from the perspective of serotonergic regulation // Research Journal of Pharmacy and Technology. – 2023. - Vol: 16, Issue: 9 (Q2, процентиль 56).
2. Akhmetova M., Nigmatullina R., Mindubayeva F., Tykezhanova G., Niyazova Y., Lepesbayeva G. Features of the effect of serotonin on the inotropic function of the right ventricular myocardium in the early postnatal period in infant rats with altered serotonin levels during their embryogenesis // Research Journal of Pharmacy and Technology - 2022. - Vol. 15, N 1. – P.122-126. DOI: 10.52711/0974-360X.2022.00020
3. AAkhmetova M., Nigmatullina R., Mindubayeva F., Tykezhanova G. The effect of adrenaline on the contractility of the right ventricular myocardium in rat pups with altered serotonin concentration in ontogenesis // RJPT. - India, 2022. - №15 (5) – P. 1-7. DOI: 10.52711/0974-360X.2022.00333
4. Mindubayeva F.A., Niyazova Yu.I., Ospanova M.D., Nigmatullina R.R. Study of serotonin metabolism in the development of pulmonary arterial hypertension in children with congenital heart defects // Abstract in the materials of the X anniversary congress “Pulmonary hypertension - 2022”: Russia, Moscow, December 5-6, 2022.
5. Mindubayeva F.A., Nigmatullina R.R., Ospanova M.D., Akhmetova M.Zh., Niyazova Yu.I. Galembikova A.R. The influence of excess serotonin in the embryonic period of ontogenesis on the activity of the membrane serotonin transporter in early postnatal ontogenesis // Abstract in the materials of the XXIV Congress of the Physiological Society named after. I.P. Pavlov: Russia, Saint-Petersburg, September 11-15, 2023
6. Mindubaуeva F.A., Ospanova M.D., Akhmaltdinova L.L., Bolatbekuly Zh. Study of serotonin metabolism in children with congenital heart defects // 34th European Pediatrics Conference: Zurich, Switzerland, October 9-10, 2023
7. Ospanova M.D., Mindubayeva F.A., Sadykova D.I. Involvement of the serotonin system in the development of pulmonary arterial hypertension // Science and Health. – 2023. - No5 (T.25). Accepted for publication

1. Scientific supervisor Atazhanova Gayane Abdulkakhimovna (born 1965) – Academician of the National Academy of Sciences of the Republic of Kazakhstan, Doctor of Economics, Professor. The Hirsch index for Scopus 9. Scopus Author ID: 6602763191.Author of more than 300 scientific papers, 30 patents of the Republic of Kazakhstan, more than 70 publications in international journals.

Microscopic and histochemical examination of the aboveground organs of Mentha arvensis, Mentha piperita, Galatella punctata, Achillea micrantha was performed.

Plant extracts have been isolated and developed by ultrasonic and microwave extraction of Tatar honeysuckle, changeable ferula, Dzhungarian ferula, Scots pine, narrow-leaved willow tea, common yarrow. Essential oils from Asian yarrow, large-flowered hyssop, Scots pine, doubtful hyssop, ziziphora odoriferous have been isolated and their chemical compositions have been identified by GC-MS method. The main ones are mono- and sesquiterpenoids such as pulegon, eucalyptol, pinene, borneol, hamazulene and bornyl acetate, etc.

Molecules of borneol, caffeic, rosemary, ferulic and gallic acids were isolated and identified by spectral methods.

The chemical composition of phenolic compounds of extracts of Tatar honeysuckle, changeable ferula, Dzhungarian ferula, Scots pine, narrow-leaved willow tea was studied using high-performance liquid chromatography (HPLC) in combination with an ultraviolet detector (UV) and tandem real-time mass spectrometry (ESI-MS/MS).

The collection of raw materials (Mentha arvensis, Mentha piperita, Galatella punctata, Achillea micrantha, Salvea dumentosa, Ferula varia, Ferula joongarica, etc.) was carried out in August 2024 on the territory of the Karaganda region. All samples of raw materials have been verified and identified by species, dried, crushed and prepared for extraction and isolation of individual compounds.

Extracts of Salvia stepposa (40% ethanol), Ferula soongarica (70% ethanol), Ferula varia (70% ethanol), as well as caffeic and ferulic acids exhibit full or almost complete (98-100%) inhibitory activity against the development of Streptococcus mutans biofilm

Inhibitory activity of 40% ethanol extract of Salvia stepposa (5, 7.5, 10 mg/ml), Prunella vulgaris gel with carbopol (5 mg/ml), 70% ethanol extract of Ferula soongarica (1, 2.5, 5 mg/ml), 70% ethanol extract of Ferula varia (1, 2.5, 5 mg/ml), 70% ethanol extract of Chamerion angustifolium (5, 7.5 mg/ml), extract of Potentilla bifurca (5 mg/ml), carbon dioxide extract of Scabiosa (2.5, 5 mg/ml), extract of Xanthium strumarium (7.5, 10 mg/ml), rosemary acid (5 mg/mlml), caffeic acid (5 mg/ml) and ferulic acid (7.5, 10 mg/ml) is significantly higher compared to the corresponding concentrations of DMSO.

As part of the study, experiments were conducted to analyze the expression of the gyrB, YycFG and MecA genes in Streptococcus mutans microorganisms under the influence of plant substances: rosemary acid, chlorogenic acid, ferulic acid and borneol. Each of these substances has demonstrated a pronounced suppressive effect on the expression of target genes, which indicates the potential effectiveness of these substances in inhibiting the processes associated with the formation and stability of biofilms. In the future, an analysis using the ΔΔCt (delta delta Ct) method will be performed to quantify changes in the level of gene expression compared to the control group.

The field of application of the expected results is pharmaceutical production technology, pharmaceutical chemistry and chemistry of natural compounds.

The target consumers of the expected results are pharmaceutical and dental companies, pharmacies, supermarkets and shops.

The results of the project in the near future may have a scientific and technical effect, in the long term - social and economic effects in reducing the risk of caries and inflammatory diseases of the oral cavity in humans.

Scientific publications (Scopus, SHEQAC) within the framework of the project, as well as monographs. Patents.

In 2024, 1 (one) article was published in Q2: Atazhanova, G.A.; Levaya, Y.K.; Badekova, K.Zh.;Ishmuratova, M.Yu.; Smagulov,M.K.; Ospanova, Zh.O.; Smagulova, E.M. Inhibition of the Biofilm Formation of Plant Streptococcus mutans// Pharmaceuticals 2024, 17, 1613. https://doi.org/10.3390/ph17121613

1 (one) article in SHEQAC: Y.K. Levaya, K.Z. Badekova, G.A. Atazhanova. Rosmarinic acid inhibits biofilm formation of Streptococcus mutans. // Bulletin of KarU, series: Biology, geography, medicine. – 2024, No. 4. – pp. 190-196. DOI 10.31489/2024BMG4/190-196

1 (one) application has been filed patent of the Republic of Kazakhstan: «1,7,7-trimethylbicyclo-[1.2.2]-heptanol-2(borneol) inhibiting the formation of Streptococcus mutans biofilm» dated No. 2024/0702.1 dated 08/27/2024

Scientific supervisor Badekova K.Zh., PhD Hirsch index is 2 according to Scopus, Scopus Author ID 57225022713. Author of more than 25 scientific papers, 6 publications in Scopus and 2 patent of the Republic of Kazakhstan.

1. Badekova K, Atazhanova G, Losseva I, Medeshova A, Aitkenova A, Brazhanova A. Acute Toxicity of Dental Gel Based on Origanum vulgare in Mice // J Toxicol., 2023. Volume 2023, Article ID 6691694, doi.org;pmc.ncbi.nlm.nih.gov
2. Badekova, K.Zh., Atazhanova, G.A., Ishmuratova, M.Yu., Brazhanova, A.K. Tazhina, A.M. Morpho-Anatomical study of Origanum vulgare L. Growing in the Akmola Region of Kazakhstan// Research Journal of Pharmacy and Technology., 2024, 17(7), 3013–3018; www.rjptonline.org
3. Badekova, K.Z. , Atazhanova, G.A., Kacergius, T., Akhmetova, S.B., Smagulov M.K. Formulation of an Origanum vulgare based dental gel with antimicrobial activity. //Journal of Taibah University Medical Sciences, 2021, 16(5), 712–718; /www.sciencedirect.com
4. Ataeva A.K., Atazhanova G.A., Badekova K.Zh. Quality assessment of essential oils using GC-MS analysis. Medicine and Ecology. - 2020. - No. 1. - P. 64-77. (KOKNVO).
5. Badekova K.Zh., Levaya Ya.K., Atazhanova G.A., Zholdasbaev M.E. Biological properties of rosmarinic acid // Pharmacy of Kazakhstan - Almaty. - 2020. - No. 7-8. P. 29-34. (KOKNVO).

Patents of the Republic of Kazakhstan:

1. Badekova K.Zh., Atazhanova G.A., Ivasenko S.A., Akhmetova S.B., Loseva I.V., Aitkenova A.A. Antimicrobial dental gel. Patent of the Republic of Kazakhstan No. 35343 dated 05.11.2021
2. Badekova K.Zh., Atazhanova G.A., Levaya Ya.K. Use of dry extract of Origanum vulgare L. (common oregano) as an antimicrobial agent. Utility model patent No. 10323 dated 30.01.2025.

Levaya Y.K., PhD Hirsch index according to Scopus – 4. Scopus Author ID 57208859362. Researcher ID Web of Science CAF-3424-2022 ORCID 0000-0003-1974-2701.

1. Levaya Ya.K., Ishmuratova M.Yu., Atazhanova G.A., Zilfikarov I.N., Loseva I.V. Microscopic evaluation of Salvia stepposa des.-schost above-ground part widespread in Kazakhstan // Research Journal of Pharmacy and Technology.- 2021.- Vol. 14(9).- P. 4773–4776. Q2 (CiteScore – 1.4). Highest percentile - 50%; 17/32 Pharmacology, Toxicology and Pharmaceutics (miscellaneous). Citations – 6 Scopus. doi.org
2. Levaya Ya.K., Zholdasbaev M.E., Atazhanova G.A., Akhmetova S.B. Evaluation of Antibacterial Activity of Salvia stepposa Extracts Isolated using Microwave Extraction, Growing Wild in Kazakhstan // Trends in sciences.- 2022.- Vol. 19(7).- P. 3217. Q3 (CiteScore – 0.8). Highest percentile - 33%; 89/134. Citations – 4 Scopus. doi.org
3. G.A. Atazhanova, K. Zh. Badekova, S.A. Ivasenko, T. Kacergius, Ya.K. Levaya, G.K. Kurmantaeva, M.Yu. Ishmuratova, M.K. Smagulov. Influence of essential oils on the formation of Streptococcus mutans biofilms// Research J. Pharm. and Tech. - 2022. – Vol. 15(11). - P. 4959 – 4966. Q2 (CiteScore – 1.4). Highest percentile - 50%; 22/43 Pharmacology, Toxicology and Pharmaceutics (miscellaneous). Citations – 5 Scopus. doi.org
4. Levaya Ya.K., Atazhanova G.A., Kacergius T., Ivasenko S.A., Marchenko A.B., Ishmuratova M.Yu., Smagulov M.K. Salvia dumetorum essential oil: GC-MS analysis, antibacterial activity and effect on the formation of Streptococcus mutans biofilms // Natural Product Research. Scopus. 2024, 38(20), 3555–3561. Q1 (CiteScore – 5.1). Highest percentile - 78%; 111/516 Plant science. Citations – 4 Scopus. doi.org
5. Atazhanova, G.; Ishmuratova, M.; Levaya, Y.; Smagulov, M.; Lakomkina, Y. The Genus Hyssopus: Traditional Use, Phytochemicals and Pharmacological Properties. Plants 2024, 13(12), 1683. Q1 (CiteScore – 6.5). Highest percentile - 86%; 96/721 Ecology, Evolution, Behavior and Systematics. Citations – 1 Scopus. doi.org
6. Levaya Ya.K., Zholdasbaev M.E., Atazhanova G.A., Akhmetova S.B., Ishmuratova M.Yu. Antibacterial activity of ultrasonic extracts of Salvia stepposa growing in Kazakhstan. Bulletin of Karaganda University. Series Biology. Medicine. Geography. - 2021. - No. 1 (101). - P. 45-49. (KOKNVO). doi.org
7. Levaya Ya.K., Atazhanova G.A. Marketing analysis of the Kazakhstani pharmaceutical market of drugs containing sage // Bulletin of KazNMU named after S.D. Asfendiyarova, 2020, No. 1, pp. 717-720. (KOKNVO)
8. Levaya Ya.K., Atazhanova G.A. Distribution of some species of Salvia stepposa Des.-shost. and Salvia sclarea L. in the Republic of Kazakhstan // Pharmacy of Kazakhstan. - Almaty. - 2019. - No. 12. (KOKNVO)

Patents of the Republic of Kazakhstan:

1. Levaya Ya.K., Atazhanova G.A. Method for obtaining a dry extract from the leaves of steppe sage, which has anti-inflammatory and antimicrobial activity. Patent for utility model No. 9790 dated 11/15/2024.
2. Badekova K.Zh., Atazhanova G.A., Levaya Ya.K. Use of dry extract of Origanum vulgare L. (common oregano) as an antimicrobial agent. Utility model patent No. 10323 dated 30.01.2025.

Zholdasbayev M.E., PhD Hirsch index is 3 according to Scopus, Researcher ID GZA-7632-2022, ORCID 0000-0001-5827-9831, Scopus Author ID 57443540800.

1. Kirillov V., Pathak A., Zholdasbayev M., Serafimovich M., Daulenova M. HPLC and GC/MS analysis of Prunus ulmifolia Franch. (syn. Aflatunia ulmifolia (Franch.) Vassilcz.) leaves growing in South-Eastern Kazakhstan // Natural Product Research, 2022. Q1 (CiteScore – 4.5). Highest percentile - 75%; 118/487 Plant Science. Citations – 1 Scopus. doi.org
2. Kirillov V., Stikhareva T., Ivashchenko A., Daulenova M., Zholdasbayev M. Expanding the knowledge about Aflatunia ulmifolia (Franch.) Vassilcz. (Rosaceae), a rare forest species of Central Asia // Botany Letters, 2022, 169(1), pp. 71–82. Q2 (CiteScore – 3.2). Highest percentile - 62%; 181/487 Plant Science. Citations – 7 Scopus.
3. Zholdasbayev, M.E.; Atazhanova, G.A.; Musozoda, S.; Poleszak, E. Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities. Pharmaceuticals 2023, 16, 1106. Q2 (CiteScore – 6.1). Highest percentile - 69%; 56/183 Pharmaceutical science. Citations – 13 Scopus. doi.org

Patents of the Republic of Kazakhstan:

1. Zholdasbaev M.E., Atazhanova G.A. Use of dry extract of Prunella vulgaris L. (common blackhead) as a cytotoxic agent. Patent No. 8611 for utility model dated 11/10/2023.

Kurmantaeva G.K. The Hirsch index is 2 according to Scopus, Scopus Author ID 58484603800.

Patents of the Republic of Kazakhstan:

1. Sabieva A., Akhmetova S.B., Atazhanova G.A., Marchenko A.B., Kurmantaeva G.K., Loseva I.V. Use of essential oil of drooping dragonhead (Dracocephalum nutans L.) as an antimicrobial agent. Patent No. 8038 for utility model dated 05.05.2023

The object of the study is tablets based on steppe sage. Tablets based on dry extract of steppe sage were developed through primary collection of raw materials, identification, production of dry extract of steppe sage in an amount sufficient to obtain tablets and assessment of the quality of the obtained materials.

The technological and pharmaceutical parameters of raw material quality have been studied. The required number of dry sage extract samples for obtaining tablets has been produced. Quality control has been carried out in accordance with ND.

The total content of phenolic acids and flavonoids and rosmarinic acid in the extract has been determined quantitatively. The content of polyphenolic compounds in the dry sage extract has been analyzed, in terms of gallic acid and rutin. Using the HPLC-MS/MS method, it has been established that the dry sage extract contains in major quantities and amounts to 6.092%. It was possible to identify caffeic acid, cynaroside, and apigenin.

The required number of tablet samples based on the dry sage extract for preclinical trials has been produced. 200 tablets have been obtained from the dry extract of sage leaves. The quality control of the obtained tablets was carried out in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan for the following parameters: average tablet weight, mass homogeneity, appearance, disintegration, crush resistance, abrasion. The total content of phenolic acids and flavonoids and rosmarinic acid in the tablets was determined quantitatively. The content of polyphenolic compounds in the obtained tablets was analyzed in terms of gallic acid and rutin. Analysis of tablets by HPLC-MS/MS containing dry extract of sage showed the presence of rosmarinic acid. A quantitative determination of the content of rosmarinic acid in the tablets was carried out, which amounted to 0.41% based on the weight of the tablet.

The above-ground part of sage was collected for further production of tablets weighing 2 kg. Identification, drying, separation of organs and grinding of raw materials were carried out.

A draft monograph on herbal medicinal raw materials was developed to simplify the further procedure for registration and inclusion of this type of raw material in the State Pharmacopoeia of the Republic of Kazakhstan. In accordance with the requirements of the Order of the Minister of Health of the Republic of Kazakhstan dated November 5, 2020 No. ҚР ДСМ-183/2020 "On approval of the rules for the development, registration, coordination, approval and introduction of amendments and additions to the State Pharmacopoeia of the Republic of Kazakhstan".

Studies were conducted to investigate the general toxic and antioxidant effects in vitro of tablets based on dry extract of steppe sage.

The general toxic effect in vitro was assessed as part of a study of the cytotoxicity of tablets containing dry extract of steppe sage, conducted using three methods on Saccharomyces cerevisiae, the protozoa species Ciliphora amoebina, and Artemia salina. The results of the experiment in three different studies confirm the absence of cytotoxic effects of the tablets on the above-mentioned test objects using the in vitro method. The results of screening the antioxidant activity of tablets containing dry extract of steppe sage, conducted using the FRAP and DPPH methods, showed an increase in optical density values compared to the control. This indicates an increase in the reducing potential and the presence of antioxidant activity of the developed tablets. It has been previously established that the highest antioxidant activity of the extract is observed at a concentration of 0.75 mg/ml (according to FRAP data), and pronounced antiradical activity is observed at a concentration of 12.5 mg/ml (according to the results of the DPPH test). Considering that each tablet contains 50 mg of extract under standard dissolution conditions (in a volume of gastric contents of about 50–100 ml), concentrations comparable or close to those effective in vitro tests are achieved. Thus, when the tablet is completely dissolved in 50 ml of medium, the extract concentration will be 1 mg/ml, which exceeds the effective level for antioxidant activity (0.75 mg/ml). Previous tests of the anti-inflammatory activity of the extract contained in the tablets under study at a dosage of 50 mg showed that the drug exhibits pronounced anti-inflammatory properties. It has been established that the anti-inflammatory activity of the tablets is due to the action of the extract. The data obtained confirm that the drug has its own anti-inflammatory activity, comparable to the action of the original plant extract, in terms of extract weight.

The scope of application of the expected results is experimental pharmacology, medicinal chemistry.

The target consumers of the expected results are research organizations engaged in clinical trials, pharmaceutical companies, and the population of the Republic of Kazakhstan.

Opportunities will be presented to influence the development of science and technology, as the mechanisms of action of a new herbal remedy in the body that does not cause allergic and cumulative reactions will be revealed. Preclinical studies will be important for specialists involved in the production of herbal remedies and the study of their safety. The results of the project will be used in the educational process on pharmaceutical production technology and pharmacology.

1. Levaya, Y., Badekova, K., Atazhanova, G., & Ivasenko, S. (2025). Pharmaceutical development and standardization of Salvia stepposa tablets.// Fundamental and Experimental Biology, 11930(3), 79–88. doi.org
2. Levaya, Y. K., Badekova, K. Z., Zholdasbayev, M. E., Kurmantayeva, G. K., Atazhanova, G. A., Sadyrbekov, D. T., & Assanali, A. (2025). Efficient Microwave-Assisted Extraction of Polyphenol-Rich Extract from Salvia dumetorum Leaves. Compounds, 5(4), 58. doi.org

1. Battakova Sharbanu Battakovna, project manager, MD, professor NJSC "Medical University of Karaganda", professor of the Department of Neurology, Psychiatry and Rehabilitation. Hirsch index 2, Scopus Author ID 56571577700, Web of Science ResearcherID NHP-9762-2025
2. Marina Archilovna Grigolashvili, PhD, associate professor. NJSC "Karaganda Medical University, Head of the Department of Neurology, Psychiatry and Rehabilitation. Hirsch index 3, ORCID: 0000-0001-6722-1762, Scopus Author ID 57193913315.
3. Shynar Sabitovna Muratbekova, Master's degree in medicine. Karaganda Medical University, Department Assistant. Hirsch index: 0, ORCID: 0000-0002-4657-4021, Scopus Author ID: 57217532868.
4. Irina Adilievna Kadyrova, Associate Professor, PhD. Email: ikadyrova@qmu.kz. Karaganda Medical University, Karaganda Research Center, Senior Researcher, Hirsch Index 7, https://orcid.org/0000-0001-7173-3138, Scopus Author ID: 57193910291.
5. Alexander Borisovich Marchenko, higher medical education, PhD. Primary place of employment and position: Karaganda Medical University, Researcher, Shared Use Laboratory, Research Center. Hirsch Index 1, ORCID: 0000-0002-0325-7659, ResearcherID: P-5367-2017, Scopus Author ID: 57193758669.
6. Mira Baikonurovna Beisembayeva, higher medical education. NJSC "Medical University of Karaganda", assistant of the Department of Neurology, Psychiatry and Rehabilitation. ORCID 0000-0001-5691-8804.
7. Mikhail Dmitrievich Mazurchak, higher medical education. Multidisciplinary Hospital named after Professor Kh.Zh. Makazhan, Head of the Center for Neurosurgery and Neurology. Neurologist of the highest category. Vice President of the Kazakhstan National Association of Neurologists NEUROSCIENCE. Chief Neurologist of the Health Department of the Karaganda Region.
8. Ivan Anatolyevich Turkevich, higher medical education, Akzhan LLP, Head of the Stroke Department.
9. Elena Valerievna Shayakhmetova, higher medical education, doctoral student 8D10100. NJSC "Karaganda Medical University," doctoral student. Hirsch index 0, ORCID: 0009-0005-5997-6061.
10. Irina Aleksandrovna Li, resident in adult and pediatric neurology. NJSC "Karaganda Medical University", resident student. H-index 0.
11. Solyanov Dmitry Andreevich, doctoral student, 8D10100. NJSC "Karaganda Medical University", research assistant.
12. Koshelyuk Alina Alekseevna, doctoral student, 8D10100. NAO "Karaganda Medical University", research assistant.
13. Bakenova Altyn Duisenkyzy, Junior Researcher, Research Laboratory of the National Agrarian University of Kazan, Researcher.

Additional staff:

1. Vera Aleksandrovna Vasyutina, second-year Master's student in Nursing (scientific and pedagogical focus). Code: 7M10103, Karaganda Medical University. Nurse.

The study groups for subsequent instrumental, laboratory, and statistical testing have been formed: 200 participants, in accordance with the inclusion and exclusion criteria. The ischemic stroke group included 50 patients, the hemorrhagic stroke group included 50 patients, the TIA group included 50 patients, and the control group included 50 patients.

Venous blood samples were collected to determine biomarkers in the study groups (the ischemic stroke group included 50 patients, the hemorrhagic stroke group included 50 patients, and the TIA group included 50 patients). Blood samples were collected twice, 3-4.5 hours and 12 hours apart, and once in the control group (50 participants). Blood plasma was isolated by centrifugation. The collected plasma samples were transferred to specialized labeled containers and stored in deep freeze until subsequent stages (for further chromatographic analysis to determine metabolite levels and PCR analysis of microRNA parameters according to the schedule). Data were collected from patient records (CT/MRI of the brain, complete blood count, coagulation profile) in the study groups.

Transcranial Doppler ultrasonography was completed in the study groups (ischemic stroke (IS) group - 50 patients, hemorrhagic stroke (HS) group - 50 patients, TIA group - 50 patients, control group - 50 patients), identifying characteristic parameters. Blood flow velocity (V ps) parameters in the IS group showed significant decreases in the affected circulation, while in the TIA group, a moderate decrease in the affected circulation was observed. HS parameters were normal or decreased depending on the affected vessel and its severity, while in the control group, parameters were within the normal range. The pulsatility index (PI) showed the most significant changes in the ischemic stroke group, normal or elevated values in the ischemic stroke and TIA groups, and within the normal range in the control group.

A chromatographic study of the metabolite levels of amino acids (Glutamate, Glutamine, Tyrosine, Phenylalanine, Valine, Lysine, Arginine, and Tryptophan) was conducted in 200 subjects (50 patients with ischemic stroke, 50 control patients, 50 patients with PI, and 50 patients with TIA).

The most pronounced changes were observed in ischemic stroke, reflecting the activation of amino acid catabolism and the development of metabolic stress. A group of patients with ischemic stroke

In the first hours of the disease (portion A), elevated levels of valine (29.9 ± 8.4 μmol/L), phenylalanine (18.3 ± 5.7 μmol/L), and glutamate (1.03 ± 0.70 μmol/L) were observed compared to the control group (valine - 22.1 ± 6.3 μmol/L, phenylalanine - 13.5 ± 3.2 μmol/L, glutamate - 0.95 ± 0.29 μmol/L).

After 12 hours (portion B), valine levels decreased moderately (to 26.8 ± 7.4 μmol/L), while tryptophan (17.1 ± 4.8 μmol/L) and arginine (0.37 ± 0.34 μmol/L) showed a tendency to increase compared to baseline values (tryptophan - 16.3 ± 4.7 μmol/L, arginine - 0.35 ± 0.45 μmol/L).

TIA patient group

Amino acid spectrum changes in TIA were less pronounced. Concentrations of most amino acids remained close to control values, with no significant changes between portions A and B.

A slight decrease in glutamate (from 1.02 ± 0.59 to 0.88 ± 0.47 μmol/L) and a more significant decrease in arginine (from 0.42 ± 0.91 to 0.24 ± 0.20 μmol/L) were observed, which may indicate the short duration of ischemia and the absence of significant and prolonged energy deficit.

Hemorrhagic Stroke Patient Group

For patients with hemorrhagic stroke, amino acid concentrations were generally closer to control values.

Average levels of valine (22.3 ± 5.4 → 21.1 ± 5.7 μmol/L), glutamate (0.68 ± 0.23 → 0.67 ± 0.25 μmol/L), and phenylalanine (14.5 ± 2.4 → 14.6 ± 3.4 μmol/L) remained relatively stable over time. A slight increase in tryptophan (from 12.4 ± 3.8 to 13.5 ± 4.0 μmol/L) can be considered a response to oxidative stress and activation of the kynurenine pathway.

In the control group, amino acid concentrations remained within physiological limits.

The work will be continued.

A partial PCR study of microRNA levels was conducted. Total free RNA was isolated from 74 clinical samples (24 control group samples, 26 IS patient samples, 12 HS patient samples, and 12 TIA patient samples). Following RNA isolation, 72 high-quality samples underwent reverse transcription and preamplification. Next, quantitative real-time PCR (qPCR) analysis of 10 microRNAs (hsa-let-7e-5p, hsa-miR-16-5p, hsa-miR-21-5p, hsa-miR-106b-5p, hsa-miR-107, hsa-miR-125a-5p, hsa-miR-125b-5p, hsa-miR-130a-3p, hsa-miR-143-3p, and hsa-miR-222-3p) was performed in 60 samples using hsa-miR-16-5p.

This work will be continued.

A panel of early cerebral ischemia biomarkers based on serum metabolites will be proposed. This panel can be used in hospitals in the Republic of Kazakhstan equipped with the appropriate laboratory equipment upon admission of patients with acute cerebrovascular diseases. Research laboratories will be involved in the further development of rapid tests.

1. Rybalkina Dina Khabibullayevna, Candidate of Medical Sciences, Professor of the Department of Internal Diseases KMU, doctor of Radiation Diagnostics, h-index (based on Scopus): 2, https://orcid.org/0000-0002-2041-1259, Researcher ID: AFF-7942-2022, Scopus Author ID: 54890642500

2. Sheveleva Nailya Igorevna, MD, Professor of the Department of Neurology, Neurosurgery, Psychiatry and Rehabilitation KMU, h-index (based on Scopus): 1, https://orcid.org/0000-0002-6658-7399, Scopus Author ID: 57193637428

3. Dyusembayeva Nailya Kamashevna, MD, Professor of the Department of Clinical Pharmacology and Evidence-based Medicine of the KMU, h-index (based on Scopus): 3, https://orcid.org/0000-0002-0555-1333, Scopus Author ID: 7801566559

4. Lyazat Kataevna Ibrayeva, Professor of the Department of Internal Diseases of the KMU, h-index (based on Scopus): 2, https://orcid.org/0000-0002-9917-0258, Scopus Author ID: 56712888900, ResearcherID: ABZ-8951-2022

5. Alina Asel Razakovna, Professor of the Department of Internal Diseases of the KMU, h-index (based on Scopus): 2, https://orcid.org/0000-0002-3229-1506, Scopus Author ID: 36967305200, ResearcherID: ABZ-7000-2022

6. Bacheva Irina Viktorovna, Associate Professor of the Department of Internal Diseases of KMU, h-index (based on Scopus): 2, https://orcid.org/0000-0002-5576-8637, Scopus Author ID: 56612421200, ResearcherID: AAR-4890-2020, SciProfiles: 3228376

7. Minbayeva Larisa Sergeevna, PhD, rehabilitation doctor, MDT of the Department of Education, National Research Center of Traumatology and Orthopedics named after Academician Batpenov N. D., Astana, h-index (based on Scopus): 1, https://orcid.org/0000-0002-8895-9878, Scopus Author ID: 57193638066

8. Bekov Yernur Kasipovich, Master of Medical Sciences, completed his doctoral studies at Karaganda Medical University, Assistant профессора Professorы of Internal Medicine Departments, cardiologist of the highest category, h-index (based on Scopus): 1, https://orcid.org/0000-0001-9422-2592, Scopus Author ID: 57196123720

9. Tulegenova Dina Yertargynovna, completedшила her doctoral studies at Медицинскогоthe Karaganda State Medical University, lecturer-researcher of the Departmentsы of Internal Diseases, endocrinologist, https://orcid.org/0000-0001-9899-619X, Scopus Author ID: 57221823692

10. Zhumagaliyeva Diana Askarovna, PhD student of the Department of Internal Diseases of the KMU, https://orcid.org/0009-0003-8028-6482

11. Ovtsynov Pavel Vladislavovich, Resident in the specialty "Adult and children's gastroenterology", Karaganda Medical University, 7th year intern, https://orcid.org/0009-0006-0450-3053

12. Aleshina Natalia Yurievna, specialist of theDepartment of Scientific Work of the KMU, h-index (based on Scopus): 1, https://orcid.org/0000-0003-4883-5894, Scopus Author ID: 57064264300

13. Danilin Alexey Olegovich, Head of the Public Procurement Department of the Cabinet of Ministers of Ukraine, Lawyer

Agreements were drawn up and signed with third-party organizations (InVivo, OLYMP) to conduct laboratory tests according to the grant project schedule. Gene polymorphisms are identified based on 100 collected genetic analyses in athletes of the Karaganda region. The database of sports achievements is filled in for 100 athletes who signed informed consent to be included in the study (hockey players, football players, handball players and martial artists, of which 30% are masters of sports, 60% are candidates for master of sports).

A literature review on gene polymorphisms in sports genomics was compiled from the Scopus and Web of Science databases for the period of 10 years (2014-2024). More than 1.5 thousand articles were found on the key designations of known gene polymorphisms associated with physical activity parameters in sports determined in clinical laboratories. We selected 50 sources, original articles, and meta-analytical reviews that are cited at least 5 times for this topic review. A database was created for 100 athletes (mean age 23.29±7.39 years) Karaganda region, who signed an informed consent to participate in the study to fill in data on gene polymorphisms (ACE RS4341, ACTN3 rs1815739, ADRB2 RS1042713, ADRB2 RS1042714, AGT rs699, AGTR2 rs11091046, AMPD1 RS17602729, APOE9358 rs429358, AQP RS1049305, BDKRB2 rs1799722, CKM rs8111989, COL1A1 rs1800012, COL5A1 rs12722, COMT rs4680, CYP1A1 rs762551, G6PC2 rs560887, GBF1 RS2273555, GDF5 rs143383, GSTM1 GSTM1, GSTT1 GSTT1, HFE RS1799945, HIF1A rs11549465, IGF1 rs35767, IL6 rs1800795, KCNJ11 rs5219, MCT1 RS1049434, MTH-FR31 rs1801131, MTH-FR33 rs1801133, NOS3 RS2070744, eNOS RS1799983, PPARA rs4253778, PPARG rs1801282, PPARG-C1A rs8192678, SOD rs4880, TRHR rs16892496, UCP2 rs660339, UCP3 rs1800849, VDR rs731236, VEGFA rs2010963, VEGFR2 rs1870377, ADRB3 rs4994, FABP2 rs1799883, FTO rs9939609, MC4R rs17782313, TCF7L2 rs12255372, TNF rs1800629, HLA-DQA1 RS2187668, LCT RS4988235, CRP RS1205, IL6R RS2228145, NRF RS7181866, MSTN rs1805086).

Advanced training in the field of sports medicine was obtained on the basis of the 1st Sechenov Moscow State Medical University of the Ministry of Health of the Russian Federation at the courses "Bioimpedance study of body composition" (Rybalkina D. Kh.), "Echocardiography in sports" (Bekov E. K.), "Sports nutrition" (Dyusembayeva N. K.).

The analysis of the relationship of gene polymorphism with sports achievements is carried out according to literature sources, a database is formed and filled in for 100 athletes taken into the study, based on their portfolio of achievements (year of receiving the category, title of cmc, ms, number of games in competitions of national and international levels, number of victories in competitions of national and international levels, number of goals scored goals, pucks). The database of athletes ' laboratory tests (UAC, CRP, ALT, AST, lactate, total protein, direct bilirubin, total bilirubin, urea, creatinine, glucose, total cholesterol, ionized calcium, testosterone, insulin, vitamin D) is filled in.

100 athletes are being examined during rest and physical activity (spirography, heart rate variability, bioimpedance measurement, densitometry, echocardiography) to assess the remodeling of the respiratory and cardiovascular systems during physical exertion, body composition.

It was found that 33.33% of athletes demonstrated a slight increase in forced vital capacity (FVC) according to the GLI-2012 Z-score, which objectively confirms a high level of physical fitness and adaptive remodeling of the respiratory system. These values exceed the average reference values for the general population and correlate with the athletic performance of the examined athletes. A statistically significant association was identified between the AGTR2 gene polymorphism and vital lung capacity (VC) (p = 0.028). Carriers of the C/C genotype exhibited higher vital capacity values compared with carriers of the A/A genotype, representing a novel finding and confirming the genetic determination of the adaptive potential of the respiratory system in athletes.

Adaptive remodeling of the cardiovascular system in athletes was assessed using the integral index of regulatory systems activity (PARS). The mean PARS value was 3.92 ± 0.22 points (95% CI: 3.53–4.30), corresponding to a state of moderate regulatory system strain. This value falls within the range typical of highly trained athletes, in whom the body mobilizes additional functional reserves to adapt to systematic physical нагрузки. The observed changes reflect physiological remodeling of the cardiovascular system in response to regular training. The degree of regulatory system strain does not exceed adaptive capacity and indicates the formation of a “sports heart”, a complex of morphofunctional adaptations that ensure high physical performance.

Statistically significant, albeit weak, positive correlations were identified between skeletal muscle parameters (SMM, FFM, SMI) and bone density measures (Z-score, BUA), with correlation coefficients of r ≈ 0.15–0.17. Multiple regression analysis was applied to construct a predictive model (Z-score ~ SMM + phase angle), demonstrating the independent contributions of skeletal muscle mass and phase angle to variability in bone mineral density. These findings may be considered when assessing osteopenia risk in individuals with different body composition profiles, as well as when evaluating the allostatic potential of athletes with distinct tissue remodeling patterns. The obtained results provide a foundation for a personalized approach to the assessment of adaptive reserves and optimization of the training process.

Statistically significant associations between genetic polymorphisms and body composition parameters were identified using the Kruskal–Wallis test, including SMM (p = 0.024), TBW (p = 0.019), ICW (p = 0.023), ECW (p = 0.014), and FFM (p = 0.019). These data form the basis for a personalized approach to nutritional support in athletes, taking genetic characteristics into account.

The identified changes in the genetic and physiological status of athletes, as well as remodeling of the cardiovascular and respiratory systems, can be applied in the planning of sports training to achieve "elite" performance. These findings may also be used to prevent potential pathological conditions requiring rehabilitation and, in the future, could be utilized in genetic therapy in medicine. Identifying gene polymorphisms in a cohort of athletes from the Karaganda region will expand knowledge about the geographical features of sports genomics.

1. Zhumagalieva D.A., Bidzhieva S.H., Sheveleva N.I., Rybalkina D.H., Fatyukhina O.A. Assessment of injury risk among handball athletes of the Karaganda region // "ANaMed Forum – New Generation 2025". 4-5.06.2025 Almaty. Kazakh National Medical University named after S.D. Asfendiyarov

2. Zhumagalieva D.A., Rybalkina D.H., Ibraeva L.K., Bacheva I.V. Analysis of polygenic profiles in employees of special purpose units in relation to the health status of the body // "ANaMed Forum – New Generation 2025". 4-5.06.2025 Almaty. Kazakh National Medical University named after S.D. Asfendiyarov

3. Rybalkina D.H., Ibraeva L.K., Bacheva I.V., Zhumagalieva D.A., Khamraeva G.B., Ovtsynov P.V. Urinary system status in athletes considering their genetic profile // Avicenna Bulletin (CQASHE, Scopus), received a certificate of acceptance for publication in issue 3, 2026.

4. Rybalkina D. K., Zhumagalieva D. A., Ibraeva L. K., Sheveleva N. I., Dyusembayeva N. K., Alina A. R., Minbaeva L. S., Bacheva I. V., Kadyrova I. A. Molecular and genetic characteristics of endurance in athletes (a case study of the Karaganda Region): methodological recommendations // Karaganda: Karaganda Medical University, 2025.-27 p.

5. Rybalkina D. K., Dyusembaeva N. K., Ibraeva L. K., Sheveleva N. I., Minbaeva L. S., Bacheva I. V., Zhumagalieva D. A., Tulegenova D. E. Molecular and genetic characteristics of injury risk in professional athletes (a case study of the Karaganda Region): methodological recommendations // Karaganda: Cabinet of Ministers of the Republic of Kazakhstan, state National Center for health and tourism of the Ministry of health of the Republic of Kazakhstan, 2025.-31 p.

6. Bidzhieva S.Kh., Zhumagalieva D.A., Sheveleva N.I. Association between bone density, mineral mass, and ionized calcium in athletes from different sports // Traumatology and Orthopaedics of Kazakhstan (CQASHE). No. 76. 2025. pp. 79-80. Special issue of the proceedings of the International Scientific and Practical Conference dedicated to the 110th anniversary of Professor H.J. Makazhanov, “Modern Approaches in Traumatology, Orthopedics, and Rehabilitation: Innovations and Practical Applications”, 9-10.2025".

7. Tokhtieva D.A., Rakhimbaeva A.N., Zhumagalieva D.A., Rybalkina D.Kh. Analysis of the relationship between endocrine status parameters and genetic polymorphism determinants in athletes // EPOMEN: Medical Sciences. 2025. No. 23. P 83-88.

8. Rybalkina D.K., Ibraeva L.K., Sheveleva N.I., Zhumagalieva D.A., Fatyukhina O.A. intellectual property No. 61976 September 9, 2025 Relevance of the predicted insolation index for reducing osteopenia risk in hockey players.

Project leader: Dr. Yemerk Meyramovich Turgunov, Doctor of Medical Sciences. Research team members: Dr. Nikita Dmitrievich Kubin, Doctor of Medical Sciences; Dr. Dmitry Vladimirovich Vazenmiller, PhD; Dr. Elena Vadimovna Avilova, Candidate of Medical Sciences; Dr. Alena Vladimirovna Lavrinenko, PhD; Leonid Viktorovich Sevastyanov, Master of Medical Sciences.

a device for non-invasive determination of the optimal length and implantation site of a urosling has been developed. A total of 88 surgical procedures for moderate to severe urinary incontinence in women have been performed. Interim analysis has been conducted comprehensively and includes: age, body mass index, symptom severity, type of urinary incontinence, characteristics of surgical intervention, anamnesis, postoperative course, quality-of-life indicators, urogenital tract microbiota, and ultrasound parameters of the urethrovesical segment. In total, 65 parameters were evaluated for each of the 88 patients, representing a substantial volume of completed research work. Data collection is ongoing.

the practical significance of the research lies in the possibility of applying the developed device in clinical practice to determine the optimal level for suburethral sling implantation. Based on the study results, an algorithm for managing patients in the postoperative period will be developed.

1. Patent application submitted for the invention “Device for non-invasive determination of urethral length and optimal level of suburethral sling implantation in women with stress and mixed urinary incontinence.” Application No. 2024/1006.1 dated 21.11.2024. A positive decision from the formal examination has been obtained.
2. One article published in a peer-reviewed national journal recommended by the Committee for Quality Assurance in Education and Science (KOKNVO): Sevastyanov L.V., Avilova Ye.V., Turgunov Ye.M., Vazenmiller D.V. Relationship between parameters of the urethrovesical segment and quality of life in women with stress urinary incontinence. Medicine and Ecology. 2024;(3):90–97. (In Russ.) doi.org
3. A literature review, “Vaginal Microbiota in Women with Stress Urinary Incontinence,” published in Issues of Gynecology, Obstetrics and Perinatology, 2025, Vol. 24, No. 5, pp. 95–101.

• The project supervisor is Musa Yerkinuly Zholdasbayev (born in 1995), PhD in the specialty 8D07201 – "Pharmaceutical Production Technology", Associate Professor at the School of Pharmacy of the National Academy of Sciences "Karaganda Medical University". Author of more than 15 scientific papers, including 7 publications in Scopus and 2 patents of the Republic of Kazakhstan. The Hirsch index is 3 according to Scopus, Researcher ID GZA-7632-2022, ORCID 0000-0001-5827-9831, Scopus Author ID 57443540800. The position in the project is the project manager.
• The executor Atazhanova Gayane Abdulkakhimovna (born in 1965) is a corresponding member. NAS RK, Doctor of Chemical Sciences, Professor. The Hirsch index for Scopus 9. Scopus Author ID: 6602763191. Author of more than 300 scientific papers, 30 patents of the Republic of Kazakhstan, more than 70 publications in international journals. She was the head and executor of grant projects and PCF: 1) Isolation, purification and identification of terpenoids from essential oils of plants (state registration No. 0114RK00215), carried out within the framework of the scientific and technical program (F.0655) "New biologically active compounds from plants and their synthetic analogues" for 2014-2016"; 2) grant of the Committee of the Ministry of Education and Science of the Republic of Kazakhstan "Creation of new medicinal substances based on polyprenolic compounds" (State registration No. 0113 RK 00394) for 2015-2017; 3) project of the Committee of the Ministry of Education and Science of the Republic of Kazakhstan "Development of effective methods of isolation and identification of new biologically active compounds from essential oils of plants" (State registration No. 0118 RK 00010) for 2018-2020 4) grant No. APP-SSG-16/0645F "Organization of the production of original scented antibacterial detergents based on essential oils" within the framework of the project "Stimulating productive innovations" for 2017-2019 (No. 0117RKK0003); 5) AP09562096 "Development of a new dental gel with antimicrobial and anti-caries action based on oregano" (No.0121RK00483).

1. The optimal composition of a wound healing agent with blackhead extract has been developed. Experimental samples of a wound healing agent with blackhead extract in various concentrations were obtained and developed to study their effect on bacterial chemotaxis, adhesion and hyaluronidase activity of clinical strains isolated from patients with purulent wounds.

2. The antimicrobial, antioxidant and anti-inflammatory activity of experimental samples of wound healing agent was studied. A selection of samples of wound healing agents has been made that exhibit relatively high wound healing activity, which inhibit the chemotaxis, adhesion, and hyaluronidase activity of opportunistic microorganisms isolated from purulent wounds, and also enhance the phagocytic activity of wound surface cells, as determined by cytological studies. The acute toxicity of the wound healing agent was studied in an in vivo experiment.

3. A technology has been developed for the production of a wound healing agent with an extract of common blackhead. Regulatory documents for obtaining a wound healing agent have been developed

4. A draft analytical regulatory document has been developed and standardization of a wound healing agent with blackhead extract has been carried out in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan (GF RK).

5. Drafts of regulatory and technical documentation have been compiled, which are recommended for implementation in pharmaceutical production.

One of the priority directions for the development of science and the pharmaceutical industry of the Republic of Kazakhstan is the search for ways to more efficiently use its own resources of wild and cultivated plant raw materials and produce original phytopreparations based on them at an affordable price, and at the same time not inferior in quality to its competing counterparts.

In addition, the implementation of the Project will contribute to:

- on a national scale, the scientific development of a young scientist, the promotion of his research career by publishing scientific results in highly rated journals and testing research results at international conferences;

- on an international scale, it will expand the opportunities of the Republic of Kazakhstan for equal scientific cooperation in the development and analysis of phytopreparations with wound-healing, antimicrobial, antioxidant and anti-inflammatory effects.

It is planned that the wound healing agent being created with high efficiency will have a moderate cost, which meets the interests of low-income strata, as well as increasing the level of protection of citizens of the Republic of Kazakhstan by contributing to public health, creating new jobs during the Project and improving the skills of young professionals.

Scientific publications within the framework of the project, as well as monographs. Patents.

• Zholdasbayev, M.E.; Atazhanova, G.A.; Musozoda, S.; Poleszak, E. Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities. Pharmaceuticals 2023, 16, 1106. Q2 (CiteScore – 6,1). Highest percentile - 69%. Citations – 6 Scopus https://doi.org/10.3390/ph16081106
• Zholdasbayev M. E., Ishmuratova M. Yu., Atazhanova G. A., Musozoda S. M., Ewa Poleszak. Histochemical Study of above-ground parts of Prunella vulgaris L., growing on the Territory of the Republic of Kazakhstan. Research Journal of Pharmacy and Technology. 2023; 16(12):5944-7. Q2 (CiteScore – 1,4). Highest percentile - 50%. Citations – 2 Scopus https://doi.org/10.52711/0974-360X.2023.00964
• Zholdasbayev M. E., Ishmuratova M. Yu., Atazhanova G.A., Musozoda S.M., Ewa Poleszak. Macro- and Microscopic Evaluation of above-ground parts of Prunella vulgaris L., growing on the Territory of the Republic of Kazakhstan. Research Journal of Pharmacy and Technology. 2024; 17(1):156-2. Q2 (CiteScore – 1,4). Highest percentile - 50%. Citations – 1 Scopus https://doi.org/10.52711/0974-360X.2024.00025

Patents

• Patent of the Republic of Kazakhstan for utility model No. 8611 dated 11/10/2023. "Application of dry extract of Prunella vulgaris L. (common blackhead) as a cytotoxic agent"
• Patent of the Republic of Kazakhstan for utility model No. 8813 dated 02.02.2024. "Application of dry extract of Prunella vulgaris L. (common blackhead) as an antimicrobial agent"

Scientific Supervisor: Levaya Ya.K., PhD. Hirsch Index (Scopus) – 4. Scopus Author ID: 57208859362. Web of Science Researcher ID: CAF-3424-2022. ORCID: 0000-0003-1974-2701.

1. Levaya Ya.K., Ishmuratova M.Yu., Atazhanova G.A., Zilfikarov I.N., Loseva I.V. Microscopic evaluation of Salvia stepposa Des.-Shost. above-ground part widespread in Kazakhstan // Research Journal of Pharmacy and Technology – 2021 – Vol. 14(9) – P. 4773–4776. Q2 (CiteScore – 1.4). Highest percentile – 50%; 22/43 in Pharmacology, Toxicology and Pharmaceutics (miscellaneous). Citations – 6 (Scopus). doi.org
2. Levaya Ya.K., Zholdasbaev M.E., Atazhanova G.A., Akhmetova S.B. Evaluation of Antibacterial Activity of Salvia stepposa Extracts Isolated using Microwave Extraction, Growing Wild in Kazakhstan // Trends in Sciences – 2022 – Vol. 19(7) – P. 3217. Q3 (CiteScore – 0.8). Highest percentile – 33%; 89/134. Citations – 4 (Scopus). doi.org
3. Atazhanova G.A., Badekova K.Zh., Ivasenko S.A., Kacergius T., Levaya Ya.K., Kurmantaeva G.K., Ishmuratova M.Yu., Smagulov M.K. Influence of essential oils on the formation of Streptococcus mutans biofilms // Research Journal of Pharmacy and Technology – 2022 – Vol. 15(11) – P. 4959–4966. Q2 (CiteScore – 1.4). Highest percentile – 50%; 22/43 in Pharmacology, Toxicology and Pharmaceutics (miscellaneous). Citations – 5 (Scopus). doi.org
4. Levaya Ya.K., Atazhanova G.A., Kacergius T., Ivasenko S.A., Marchenko A.B., Ishmuratova M.Yu., Smagulov M.K. Salvia dumetorum essential oil: GC-MS analysis, antibacterial activity and effect on the formation of Streptococcus mutans biofilms // Natural Product Research – 2024 – Vol. 38(20) – P. 3555–3561. Q1 (CiteScore – 5.1). Highest percentile – 78%; 111/516 in Plant Science. Citations – 4 (Scopus). doi.org
5. Atazhanova G., Ishmuratova M., Levaya Y., Smagulov M., Lakomkina Y. The Genus Hyssopus: Traditional Use, Phytochemicals and Pharmacological Properties // Plants – 2024 – Vol. 13(12) – Article 1683. Q1 (CiteScore – 6.5). Highest percentile – 86%; 96/721 in Ecology, Evolution, Behavior and Systematics. Citations – 1 (Scopus). doi.org

Patents of the Republic of Kazakhstan:

1. Levaya Ya.K., Atazhanova G.A. Method for obtaining a dry extract from Salvia stepposa leaves with anti-inflammatory and antimicrobial activity. Utility model patent №9790 dated 15.11.2024
2. Badekova K.Zh., Atazhanova G.A., Levaya Ya.K. Use of dry extract of Origanum vulgare L. as an antimicrobial agent. Utility model patent №10323 dated 30.01.2025

Scientific Consultant: Atazhanova Gayane Abdulkhakimovna – Doctor of Chemical Sciences, Professor. Hirsch Index (Scopus) – 10. Scopus Author ID: 6602763191. Author of more than 300 scientific works, 30 patents of the Republic of Kazakhstan, and over 70 publications in international journals.

1. Atazhanova G.A., Sabiyeva A., Akhmetova S., Smagulov M., Medeshova A., Sarsembayeva S., Sarsembayeva S., Aldabayeva U., Kurmantayeva G. Component Composition and Antimicrobial Activity of Dracocephalum nutans L. Essential Oil// Research J. Pharm. and Tech. – 2023. -16(10). Q3 (CiteScore – 1,3). Highest percentile - 56%. Citations – 3. DOI:10.52711/0974-360X.2023.00791. rjptonline.org
2. Zholdasbayev, M.E.; Atazhanova, G.A.; Musozoda, S.; Poleszak, E. Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities. Pharmaceuticals 2023, 16, 1106. Q2 (CiteScore – 4,7). Highest percentile - 59%. Citations – 13 Scopus doi.org;
3. G.А. Atazhanova, K.Zh. Badekova, S.А. Ivasenko, Т. Kacergius, Ya.К. Levaya, G.К. Kurmantaeva, M.Yu. Ishmuratova, М.K.Smagulov. Influence of Essential Oils on the Formation of Streptococcus mutans Biofilms// Research J. Pharm. and Tech. -2022.- 15(11). – Р. 4959-4966. Q3 (CiteScore – 0,7). Highest percentile - 56%. Citations – 5. rjptonline.org;PID=2022-15-11-19
4. Atazhanova, G.A.; Levaya, Y.K.; Badekova, K.Z.; Ishmuratova, M.Y.; Smagulov, M.K.; Ospanova, Z.O. Inhibition of the Biofilm Formation of Plant Streptococcus mutans.// Pharmaceuticals 2024, 17, 1613.https://doi.org/10.3390/ph17121613 www.mdpi.com

Patents of the Republic of Kazakhstan:

1. Badekova K.Zh., Atazhanova G.A., Ivasenko S.A., Akhmetova S.B., Loseva I.V., Aitkenova A.A. Antimicrobial dental gel. Patent of the Republic of Kazakhstan №35343 dated 05.11.2021
2. Zholdasbaev M.E., Atazhanova G.A. Use of dry extract of Prunella vulgaris L. as a cytotoxic agent. Utility model patent №8611 dated 10.11.2023
3. Sabiyeva A., Akhmetova S.B., Atazhanova G.A., Marchenko A.B., Kurmantayeva G.K., Loseva I.V. Use of essential oil of Dracocephalum nutans L. as an antimicrobial agent. Utility model patent №8038 dated 05.05.2023

The object of the study is Salvia stepposa Des.-Shost. Diagnostic features of Salvia stepposa have been identified at the macro-, micro-, and histochemical levels. Quality parameters and raw material standards have been established. A total of 30 extracts of Salvia stepposa were obtained using percolation, ultrasonic, and microwave extraction methods with various solvents (water and hydroalcoholic solutions). Extraction parameters were determined, and the component composition of the extracts was analyzed using TLC and HPLC-UV methods. A promising extract of Salvia stepposa was selected, on the basis of which tablets were developed. It was proven that the use of microwave activation for obtaining the extract reduced the extraction time by 15 times compared to other methods.

A new method for obtaining dry extract of steppe sage has been developed, which, thanks to the use of microwave extraction, is characterized by high productivity of the technological process, low consumption of extractant, and the elimination of labor-intensive and time-consuming procedures, making it accessible, rational, and economical. Quality specifications for the extract have been developed. Studies have been conducted on the biological activity, cytotoxicity, and acute toxicity of the extract. Pharmaceutical development of tablets based on extract has been carried out. The effectiveness of sage extract as an antioxidant, anti-inflammatory, and antimicrobial agent has been confirmed. A new drug based on steppe sage extract has been developed, which has no analogues on the pharmaceutical market of Kazakhstan, as it is made from raw materials grown in Kazakhstan. The developed tablets contain more rosmarinic acid than the currently existing analogues.

The significance of this work on an international scale lies in the application of modern methods for obtaining biologically active compounds from Salvia stepposa, which grows in the territory of the Republic of Kazakhstan and possesses valuable practical properties. Specifically, microwave and ultrasonic activation methods were used, which will undoubtedly contribute to the development of science and technology in Kazakhstan.

The innovative and competitive advantage of this work is associated with the development and implementation of an import-substitution system for vital pharmaceutical products for the Republic of Kazakhstan. The use of advanced technologies in the creation of new pharmacologically active substances will significantly increase production efficiency and reduce production costs.

1. Levaya, Y. К., Atazhanova, G. А., Kacergius, Т., Ivasenko, S. А., Marchenko, A. B., Ishmuratova, M. Y., & Smagulov, М. К. (2023). Salvia dumetorum essential oil: GC-MS analysis, antibacterial activity and effect on the formation of Streptococcus mutans biofilms.// Natural Product Research, 38(20), 3555–3561. Q1. Highest percentile - 78%; doi.org
2. Levaya, Y.; Atazhanova, G.; Gabe, V.; Badekova, K. A Review of Botany, Phytochemistry, and Biological Activities of Eight Salvia Species Widespread in Kazakhstan.// Molecules 2025, 30, 1142. doi.org
3. Levaya Ya.K., Atazhanova G.A. Method for obtaining dry extract from the leaves of Salvia stepposa with anti-inflammatory and antimicrobial activity. Utility model patent No. 9790 dated 15.11.2024.

The scientific supervisor is Karakoz Zailovna Badekova (born in 1985), PhD in the specialty 6D074800 – "Pharmaceutical Production Technology", Associate Professor at the Department of Informatics and Biostatistics of the National Academy of Sciences "Karaganda Medical University". The Hirsch index is 2 according to Scopus, Ssris Author ID 57225022713. Researcher ID ABC-8591-2020, ORCID 0000-0003-2736-8633.

Author of more than 25 scientific papers, 5 publications in Scopus and 1 patent of the Republic of Kazakhstan. She was the responsible executor of the scientific project "Development of a new dental gel of antimicrobial and anti-caries action based on oregano" (contract No. 256/12-2 dated June 15, 2021)., 2021-2022. He is the head of the following scientific project: "Development of a new dental product based on Origanum vulgare L.", included in the program of fundamental research of young scientists "Zhas galim" for 2022-2024.

• 1. Badekova K.Zh., Atazhanova G.A., Kacergius T., Akhmetova S.B., Smagulov M.K. Formulation of an Origanum vulgare based dental gel with antimicrobial activity. Journal of Taibah University Medical Sciences.– 2021. – Vol. 16, No 5. – P. 712-718. Q2 (CiteScore – 3,1). Highest percentile - 68%; Citations – 7 Scopus http://doi.org/10.1016/j.jtumed.2021.05.009
• 2. Badekova K.Zh., Atazhanova G., Levaya Ya.K., Kacergius T., Akhmetova S.B, Smagulov M.K. Anti-inflammatory activity of a dental gel based on Origanum vulgare raw material. Bulletin of Karaganda University. Series of Biology, Medicine, Geography. - 2021.- Vol.4.- P.91 – 96. https://dist.buketov.edu.kz/apart/2021-104-4/11.pdf (КОКНВО)
• 3. Atazhanova G.A., Badekova K.Zh., Ivasenko S.A., Kacergius T., Levaya Ya.K., Kurmantaeva G.K., Ishmuratova M. Yu., Smagulov M.K. Influence of essential oils on the formation of Streptococcus mutans biofilms // Research J. Pharm. and Tech. - 2022. – Vol. 15(11). - P. 4959 – 4966. Q3 (CiteScore – 1,3). Highest percentile - 48%; 17/32 Pharmacology, Toxicology and Pharmaceutics (miscellaneous). Citations – 1 Scopus https://doi.org/10.52711/0974-360X.2022.00834
• 4. Karakoz Badekova, Gayane Atazhanova, Irina Losseva, Aigul Medeshova, Ailazzat Aitkenova, Anar Brazhanova, "Acute Toxicity of Dental Gel Based on Origanum vulgare in Mice", Journal of Toxicology, vol. 2023, Article ID 6691694, 6 pages, 2023. Q2. Highest percentile - 64%; Citations – 0 Scopus https://doi.org/10.1155/2023/6691694
• 5. Badekova K.Zh., Atazhanova G.A., Ivasenko S. A., Akhmetova S. B., Loseva I. V., Aitkenova A. A. Antimicrobial dental gel. Patent of the Republic of Kazakhstan No. 35343 dated 05.11.2021
• 6. Atazhanova, G. A., Levaya, Y. K., Badekova, K. Zh., Ishmuratova, M. Y., Smagulov, M. K., Ospanova, Z. O., & Smagulova, E. M. (2024). Inhibition of the Biofilm Formation of Plant Streptococcus mutans. Pharmaceuticals, 17(12), 1613. Q2 (Highest percentile - 69%). https://doi.org/10.3390/ph17121613

The project executor is Gayane Abdulkakhimovna Atazhanova (born in 1965), Academician of the National Academy of Sciences of the Republic of Kazakhstan, Doctor of Chemical Sciences, Professor. The Hirsch index for Scopus 9. Scopus Author ID: 6602763191.Author of more than 300 scientific papers, 30 patents of the Republic of Kazakhstan, more than 70 publications in international journals.

• 1. Atazhanova, G.A.; Levaya, Y.K.; Badekova, K.Z.; Ishmuratova, M.Y.; Smagulov, M.K.; Ospanova, Z.O. Inhibition of the Biofilm Formation of Plant Streptococcus mutans.// Pharmaceuticals 2024, 17, 1613. https://doi.org/10.3390/ph17121613, https://www.mdpi.com/1424-8247/17/12/1613
• 2. Atazhanova, G.; Ishmuratova, M.; Levaya, Y.;Smagulov, M.; Lakomkina, Y. The Genus Hyssopus: Traditional Use, Phytochemicals and Pharmacological Properties.// Plants 2024, 13, 1683. https://doi.org/10.3390/plants13121683; https://www.mdpi.com/2223-7747/13/12/1683
• 3. Atazhanova G.A., Sabiyeva A., Akhmetova S., Smagulov M., Medeshova A., Sarsembayeva S., Sarsembayeva S., Aldabayeva U., Kurmantayeva G. Component Composition and Antimicrobial Activity of Dracocephalum nutans L. Essential Oil// Research J. Pharm. and Tech. – 2023. -16(10). Q3 (CiteScore – 1,3). Highest percentile - 56%. Citations – 0. DOI:10.52711/0974-360X.2023.00791. https://rjptonline.org/AbstractView.aspx?PID=2023-16-10-61
• 4. Zholdasbayev, M.E.; Atazhanova, G.A.; Musozoda, S.; Poleszak, E. Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities. Pharmaceuticals 2023, 16, 1106. Q2 (CiteScore – 4,7). Highest percentile - 59%. Citations – 0 Scopus https://doi.org/10.3390/ph16081106;
• 5. G.А. Atazhanova, K.Zh. Badekova, S.А. Ivasenko, Т. Kacergius, Ya.К. Levaya, G.К. Kurmantaeva, M.Yu. Ishmuratova, М.K.Smagulov. Influence of Essential Oils on the Formation of Streptococcus mutans Biofilms// Research J. Pharm. and Tech. -2022.- 15(11). – Р. 4959-4966. Q3 (CiteScore – 0,7). Highest percentile - 56%. Citations – 1. https://rjptonline.org/HTMLPaper.aspx?Journal=Research%20Journal%20of%20Pharmacy%20and%20Technology;PID=2022-15-11-19
• 6. Atazhanova, G. A., Levaya, Y. K., Badekova, K. Z., Ishmuratova, M. Y., Smagulov, M. K., Ospanova, Z. O., & Smagulova, E. M. (2024). Inhibition of the Biofilm Formation of Plant Streptococcus mutans. Pharmaceuticals, 17(12), 1613. Q2 (Highest percentile - 69%). https://doi.org/10.3390/ph17121613

A mild dosage form in the form of a gel has been developed based on pharmaceutical substances (essential oil and dry extract of Origanum vulgare L.). Antimicrobial and anti-caries effects have been proven.

A technology for obtaining a dental gel with essential oil and oregano extract has been developed.

Quality indicators and standardization of dental gel have been developed.

According to the results of preclinical (non-clinical) tests, the gel has no toxic properties and is recommended as an antimicrobial and anti-caries agent.

Pharmaceutical production technology. The final product of the project is an original medicinal product dental gel with essential oil and extract of oregano with anti-carious and antimicrobial effects, which has no analogues in the world and in Kazakhstan.

Scientific publications within the framework of the project, as well as monographs. Patents.

• Karakoz Badekova, Gayane Atazhanova, Irina Losseva, Aigul Medeshova, Ailazzat Aitkenova, Anar Brazhanova, "Acute Toxicity of Dental Gel Based on Origanum vulgare in Mice", Journal of Toxicology, vol. 2023, Article ID 6691694, 6 pages, 2023. Q2. Highest percentile - 64%; https://doi.org/10.1155/2023/6691694
• Badekova K. Zh., Atazhanova G. A., Ishmuratova M. Yu., Akhmetova S.B., Brazhanova A.K., Tazhina A. M. «Morpho - anatomical study of Origanum vulgare L. growing in the Akmola region of Kazakhstan», Research Journal of Pharmacy and Technology, 2024; 17(7):3013-8. doi: 10.52711/0974-360X.2024.00471

Patents of the Republic of Kazakhstan:

• Badekova K.Zh., Atazhanova G.A., Ivasenko S. A., Akhmetova S. B., Loseva I. V., Aitkenova A. A. Antimicrobial dental gel. Patent of the Republic of Kazakhstan No. 35343 dated 05.11.2021.
• Zholdasbaev M.E., Atazhanova G.A. The use of dry extract of Prunella vulgaris L. (common blackhead) as a cytotoxic agent. Patent No. 8611 for a utility model dated 11/10/2023.
• Sabieva A., Akhmetova S.B., Atazhanova G.A., Marchenko A.B., Kurmantayeva G.K., Loseva I.V. Application of essential oil of drooping snakehead (Dracocephalum nutans L.) as an antimicrobial agent. Patent No. 8038 for a utility model dated 05.05.2023.

• Okassov Didar Bekezhanovich, Author ID в Scopus - 57541374300, Researcher ID Web of Science - 324324, ORCID ID - 0000-0002-1575-9277
• Ivasenko Svetlana Alexandrovna, Author ID в Scopus - 6506096984, ORCID ID - 0000-0003-3074-57190

In the course of the project, for the first time, experimental samples of extracts from thyme grass were obtained using traditional and modern methods of extraction, using various extractants, and the composition of the obtained extracts was studied by HPLC-UV, HPLC-MS (a total of 6 samples).

For the first time, in an in vitro experiment, it was found that dry aqueous extract of thyme, obtained by remaceration, showed the best antitumor effect against human prostate cancer cells PC-3, while it does not affect the viability of healthy human prostate cells, also has pronounced antiradical (DPPH) activity in vitro and anti-inflammatory activity in vivo It is recommended for the development of a new drug.

For the first time, it has been established that a new herbal drug based on dry water thyme leaf extract significantly affects the PC-3 prostate cancer cell line, causing apoptosis, temporarily activating the PI3K/Akt pathway, and not significantly affecting the MAP kinase pathway. The data obtained indicate that dry water extract of thyme common-leaved has a potential therapeutic value in combating the survival of cancer cells and inducing their death.

For the first time, the technology of obtaining a new drug with antitumor effect was developed and implemented. Разработаны и утверждены Laboratory regulations for obtaining and pilot-industrial regulations for the production of medicinal products of plant origin with antitumor effect have been developed and approved. A draft normative document (ND) regulating the quality of a medicinal product of plant origin that has an antitumor effect has been developed. Pilot production has been organized and experimental batches of the drug have been obtained for further pharmacological tests.

Pharmacy, pharmaceutical production technology, pharmacology.

Patent for invention:

For 2023

• Application No. 197392 for the patent of the Republic of Kazakhstan for an invention dated 03/07/2023. A method for obtaining a drug with a cytotoxic effect // Ivasenko S.A., Okasov D.B., Atazhanova G.A., Edilbayeva T.T.
• Application No. 201002 for the Eurasian Patent for invention, registration number of application 2023/020 dated 03/24/2023. A method for obtaining a drug with a cytotoxic effect // Ivasenko S.A., Okasov D.B., Atazhanova G.A., Edilbaeva T.T.

For 2024

• Okasov D., Ivasenko S., Seité Р., Vannier B., Marchenko A., Ishmuratova M., Akhmetova S. Study of the composition of phenolic compounds, cytotoxic effect and antioxidant potential of Thymus crebrifolius Klok. extracts // Pharmaceutics. – 2024. – Vol.11. - submitted.
• Okasov D., Ivasenko S., Seité Р., Vannier B., Marchenko A., Ishmuratova M., Akhmetova S. Cytotoxicity and anticancer activity of plants of the genus Thymus L. // Pharmaceuticals. – 2024. – Vol.11. - submitted.

Scientific Supervisor – Irina Adilyevna Kadyrova, PhD, Associate Professor.

Expertise: PCR, Sanger sequencing, ELISA, statistical analysis, basic bioinformatics (genotyping, phylogenetics), research project management.

H‑index (Scopus): 9

ORCID: 0000‑0001‑7173‑3138

Scopus Author ID: 0000‑0001‑7173‑3138

Web of Science H‑index: 5

Experience: PI of 1 project, contributor to 7 projects.

IELTS: 7.0.

Dinara Meiramovna Turebekova – Junior Researcher, MSc in Biology (Research), born 2000, responsible researcher.

Experience: microbiology, molecular genetics, preclinical studies. Prizewinner of the 2021 international scientific conference for young scientists.

Shynar Sabitovna Muratbekova – neurologist of the 1st category, MSc, Assistant Lecturer at NJSC “Karaganda Medical University”.

H‑index: 0

ORCID: 0000‑0002‑4657‑4021

Scopus Author ID: 57217532868

Experience: 12+ years in neurology, 7 years teaching, 8 publications, 2 copyright certificates.

IELTS: 5.5.

Role: methodology, sample collection, chromatography, data processing, manuscript preparation.

Dmitry Andreevich Solyanov – Junior Researcher, Research Laboratory of Infectious Diseases and Immunology, NJSC “KMU”.

H‑index: 1

ORCID: 0009‑0002‑9779‑197

Education: MSc in Pharmaceutical Manufacturing Technology; Master‑2 at Sorbonne Paris Nord University.

Skills: cell culture, HPLC, RNA sequencing, qPCR, Western blotting.

Venous blood samples (n=60) were collected across four groups (control, case, comparison, second comparison). Clinical examination and questionnaire performed. SOPs developed and implemented in the research laboratory.

Metabolite levels:

TMAO (case): 0.94 μmol/L [0.55; 2.09]

TMAO (control): 0.29 μmol/L [0.14; 0.39]

5‑HIAA (case): 0.03 μmol/L [0.02; 0.04]

5‑HIAA (control): 0.04 μmol/L [0.03; 0.06]

Tryptophan (case): 9.82 μmol/L [6.07; 12.47]

Tryptophan (control): 12.98 μmol/L [11.46; 15.98]

Kynurenine (case): 0.4144 μmol/L [0.38; 0.45]

Kynurenine (control): 0.46 μmol/L [0.42; 0.56]

Neurologists, infectious disease specialists, laboratory physicians, pulmonologists, and rehabilitation specialists.

One article published in “Medicine and Ecology”, included in KOKNVO List 2:

Kadyrova I.A., Turebekova D.M., Marchenko A.B., Solyanov D.A. Contribution of dysregulation of tryptophan and trimethylamine‑N‑oxide to the molecular mechanisms of Long COVID‑19 development depending on CT stage of lung involvement// Medicine and Ecology. – 2025. - № (3). – Р. 90‑98. DOI: 10.59598/ME‑2305‑6053‑2025‑116‑3‑90‑98.

Сабиева Асель - project manager.

Scopus ID: 57208403517

ResearcherID: LPP-4084-2024

ORCID: https://orcid.org/0000-0002-6268-8977

Gayane Abdulkakhimovna Atazhanova is a scientific consultant.

Scopus ID: 6602763191 https://www.scopus.com/authid/detail.uri?authorId=6602763191

Researcher ID: AFJ-9349-2022, https://www.webofscience.com/wos/author/record/3687001

ORCID: 0000-0003-1615-9967, https://orcid.org/0000-0003-1615-9967

An extract from Dracocephalum ruyschiana was obtained using ultrasonic treatment. The aerial and underground parts of Dracocephalum ruyschiana were collected. A pharmacognostic study of the plant raw material of Dracocephalum ruyschiana was carried out, and its histochemical, anatomical, and morphological characteristics were determined.

Biomedical materials, biologically active substances, biological and medical preparations. Medicine and healthcare, general medicine, pharmacology, and pharmacy.

Yedrissov Azamat Tirzhanovich – Project Leader, Doctor of Chemical Sciences, Professor of the School of Pharmacy (h-index = 5);

Khrustalev Dmitriy Petrovich – Principal Researcher, Doctor of Chemical Sciences, Professor of the School of Pharmacy (h-index = 6);

Losseva Irina Viktorovna – Leading Researcher, Candidate of Biological Sciences, Professor of the School of Pharmacy (h-index = 3);

Vorontsova Oxana Yuryevna – Leading Researcher, Candidate of Chemical Sciences (h-index = 2);

Khrustaleva Anastassia Andreevna – Researcher, 2nd-year PhD student of the School of Pharmacy, Master of Engineering Sciences (h-index = 4);

Savelyev Artyom Fyodorovich – Junior Researcher, 1st-year PhD student of the School of Pharmacy, Master of Engineering Sciences;

Kazantsev Vladimir Vitalyevich – Junior Researcher, Master of Engineering Sciences, Lecturer at the School of Pharmacy;

Rusyaeva Polina Yuryevna – Engineer, Master’s student of the School of Pharmacy;

Kiikbayev Marlen Armanovich – Engineer, Master’s student of the School of Pharmacy.

An environmentally friendly and economically viable technology for producing bioactive and biodegradable polymer–fabric composites based on polylactic acid and natural fabrics has been developed. Bioactive substances exhibiting osteostimulating and antimicrobial activity were incorporated into certain composite formulations.

The optimal composition of the composites was determined, and samples were prepared for testing.

Samples of the studied composites were fabricated using injection molding and melt processing techniques.

Comprehensive mechanical testing of all composites was conducted.

Fourier-transform infrared (FTIR) analysis was performed to determine the chemical composition of the samples and to identify structural changes in the composites and fillers.

Thermogravimetric analysis (TGA) was carried out, providing data on the thermal stability and resistance of the samples. Dielectric and electrophysical measurements of the composites were also performed.

The consumers of the developed technology will include industrial companies manufacturing electronic printed circuit boards, as well as pharmaceutical and medical enterprises in Kazakhstan and the CIS countries. In the case of establishing independent production of products based on PLA composites, both legal entities and individuals in the Republic of Kazakhstan and the CIS countries may act as consumers.